Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion

被引：38

作者：

Lin, Xihua ^{[1
]}

Yang, Bin ^{[1
]}

Liu, Wei ^{[1
]}

Tan, Xiaochao ^{[3
]}

Wu, Fan ^{[1
]}

Hu, Peishan ^{[1
]}

Jiang, Tao ^{[2
]}

Bao, Zhaoshi ^{[2
]}

Yuan, Jiangang ^{[1
]}

Qiang, Boqin ^{[1
]}

Peng, Xiaozhong ^{[1
]}

Han, Wei ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Inst Basic Med Sci, Peking Union Med Coll, Sch Basic Med, Beijing 100730, Peoples R China

[2] Capital Med Univ, Beijing Tiantan Hosp, Dept Neurosurg, Beijing, Peoples R China

[3] Univ Texas MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Dept Thorac, Houston, TX 77030 USA

来源：

ONCOTARGET | 2016年 / 7卷 / 15期

基金：

中国国家自然科学基金;

关键词：

PCBP2; ARHGDIA; miRNA; glioma; migration and invasion; EPITHELIAL-MESENCHYMAL TRANSITION; RNA-BINDING PROTEINS; RHO-GTPASES; MESSENGER-RNA; TUMOR PROGRESSION; PROSTATE-CANCER; MICRORNA; FAMILY; GROWTH; ALPHA;

D O I：

10.18632/oncotarget.6869

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

RNA-RNAand protein-RNAinteractions are essential for post-transcriptionalregulationin normal development and may be deregulated in cancerinitiation and progression. The RNA-binding proteinPCBP2, an oncogenic protein in human malignant gliomas, is an essential regulator of mRNA and miRNA biogenesis, stability and activity.Here, we identified Rho GDP dissociation inhibitor a (ARHGDIA) as a target mRNA that binds to PCBP2, and we uncovered the role of ARHGDIA as a putative metastasis suppressor through analyses of in vitro and in vivo models of EMT and metastasis. Furthermore, we demonstrated that ARHGDIA is a potential target of miR-151-5p and miR-16 in gliomas. The interaction between PCBP2 and the 3'UTR of the ARHGDIA mRNA may induce a local change in RNA structure that favors subsequent binding of miR-151-5p and miR-16, thus leading to the suppression of ARHGDIA expression. PCBP2 may facilitate miR-151-5p and miR-16 promotion of glioma cell migration and invasion through mitigating the function of ARHGDIA.

引用

页码：19483 / 19498

页数：16

共 48 条

[31] Iron Homeostasis Regulates the Activity of the MicroRNA Pathway through Poly(C)-Binding Protein 2 [J].

Li, Yujing ;

Lin, Li ;

Li, Zigang ;

Ye, Xuecheng ;

Xiong, Ke ;

Aryal, Baikuntha ;

Xu, Zihui ;

Paroo, Zain ;

Liu, Qinghua ;

He, Chuan ;

Jin, Peng .

CELL METABOLISM, 2012, 15 (06) :895-904

[32] MicroRNA: Biogenesis, Function and Role in Cancer [J].

MacFarlane, Leigh-Ann ;

Murphy, Paul R. .

CURRENT GENOMICS, 2010, 11 (07) :537-561

[33] A NOVEL RAS-RELATED GENE FAMILY [J].

MADAULE, P ;

AXEL, R .

CELL, 1985, 41 (01) :31-40

[34] Identification and Functional Characterization of microRNAs Involved in the Malignant Progression of Gliomas [J].

Malzkorn, Bastian ;

Wolter, Marietta ;

Liesenberg, Franziska ;

Grzendowski, Michael ;

Stuehler, Kai ;

Meyer, Helmut E. ;

Reifenberger, Guido .

BRAIN PATHOLOGY, 2010, 20 (03) :539-550

[35] A Systematic Review of MicroRNA in Glioblastoma Multiforme: Micro-modulators in the Mesenchymal Mode of Migration and Invasion [J].

Moller, Heidi G. ;

Rasmussen, Andreas P. ;

Andersen, Hjalte H. ;

Johnsen, Kasper B. ;

Henriksen, Michael ;

Duroux, Meg .

MOLECULAR NEUROBIOLOGY, 2013, 47 (01) :131-144

[36] Control of tumor and microenvironment cross-talk by miR-15a and miR-16 in prostate cancer [J].

Musumeci, M. ;

Coppola, V. ;

Addario, A. ;

Patrizii, M. ;

Maugeri-Sacca, M. ;

Memeo, L. ;

Colarossi, C. ;

Francescangeli, F. ;

Biffoni, M. ;

Collura, D. ;

Giacobbe, A. ;

D'Urso, L. ;

Falchi, M. ;

Venneri, M. A. ;

Muto, G. ;

De Maria, R. ;

Bonci, D. .

ONCOGENE, 2011, 30 (41) :4231-4242

[37] Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing [J].

Newman, Martin A. ;

Thomson, J. Michael ;

Hammond, Scott M. .

RNA, 2008, 14 (08) :1539-1549

[38] Biotinylated tags for recovery and characterization of ribonucleoprotein complexes [J].

Penalva, LOF ;

Keene, JD .

BIOTECHNIQUES, 2004, 37 (04) :604-+

[39] Anti-RhoA and anti-RhoC siRNAs inhibit the proliferation and invasiveness of MDA-MB-231 breast cancer cells in vitro and in vivo [J].

Pillé, JY ;

Denoyelle, C ;

Varet, J ;

Bertrand, JR ;

Soria, J ;

Opolon, P ;

Lu, H ;

Pritchard, LL ;

Vannier, JP ;

Malvy, C ;

Soria, C ;

Li, H .

MOLECULAR THERAPY, 2005, 11 (02) :267-274

[40] αvβ8 integrin interacts with RhoGDI1 to regulate Rac1 and Cdc42 activation and drive glioblastoma cell invasion [J].

Reyes, Steve B. ;

Narayanan, Anjana S. ;

Lee, Hye Shin ;

Tchaicha, Jeremy H. ;

Aldape, Kenneth D. ;

Lang, Frederick F. ;

Tolias, Kimberly F. ;

McCarty, Joseph H. .

MOLECULAR BIOLOGY OF THE CELL, 2013, 24 (04) :474-482

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