Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing

被引:69
作者
Bachhuber, Franziska [1 ]
Huss, Andre [1 ]
Senel, Makbule [1 ]
Tumani, Hayrettin [1 ,2 ]
机构
[1] Univ Hosp Ulm, Dept Neurol, Ulm, Germany
[2] Specialty Hosp Neurol Dietenbronn, Schwendi, Germany
关键词
OLIGOCLONAL IGG; IMMUNOGLOBULINS; CAPILLARY; PROTEINS; SYSTEM; BANDS; MS;
D O I
10.1038/s41598-021-89514-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Tear fluid is receiving growing attention as a source for novel diagnostic biomarkers. Multiple techniques are available for its collection and impact the composition of acquired samples. We sought to provide a direct comparison of two collection methods with regard to implementation, acceptance, and impact on sample composition. Tear fluid was collected from fifteen healthy volunteers with capillary tubes and Schirmer strips and analyzed for total protein and IgG concentrations. Sampling parameters and perception by test persons were compared. The use of capillary tubes was more convenient for the participants while causing more effort for the collector. Tear flow rates as well as the relative and absolute amount of IgG were higher when Schirmer strips were used. Consecutive collections with Schirmer strips significantly influenced tear flow rates, IgG, and protein concentrations. A moderate correlation was observed between tear flow rates and IgG concentrations for both methods. Samples collected with both methods can be analyzed by isoelectric focusing, a potential diagnostic application in the field of neurology. The specific advantages and limitations of tear fluid sampling with either capillary tubes or Schirmer strips demonstrate the need for a thorough investigation of collection methods with regard to the application of interest.
引用
收藏
页数:9
相关论文
共 39 条
[1]   Single unit filter-aided method for fast proteomic analysis of tear fluid [J].
Aass, Cecilie ;
Norheim, Ingrid ;
Eriksen, Erik Fink ;
Thorsby, Per M. ;
Pepaj, Milaim .
ANALYTICAL BIOCHEMISTRY, 2015, 480 :1-5
[2]   Proteomic analysis of tear fluid reveals disease-specific patterns in patients with Parkinson's disease - A pilot study [J].
Boerger, Matthias ;
Funke, Sebastian ;
Leha, Andreas ;
Roser, Anna-Elisa ;
Wuestemann, Ann-Katrin ;
Maass, Fabian ;
Bahr, Mathias ;
Grus, Franz ;
Lingor, Paul .
PARKINSONISM & RELATED DISORDERS, 2019, 63 :3-9
[3]   Tear analysis in clinically isolated syndrome as new multiple sclerosis criterion [J].
Calais, Gauthier ;
Forzy, Gerard ;
Crinquette, Charlotte ;
Mackowiak, Alexandre ;
de Seze, Jerome ;
Blanc, Frederic ;
Lebrun, Christine ;
Heinzlef, Olivier ;
Clavelou, Pierre ;
Moreau, Thibault ;
Hennache, Bernadette ;
Zephir, Helene ;
Verier, Albert ;
Neuville, Veronique ;
Confavreux, Christian ;
Vermersch, Patrick ;
Hautecoeur, Patrick .
MULTIPLE SCLEROSIS, 2010, 16 (01) :87-92
[4]  
Choy CKM, 2001, INVEST OPHTH VIS SCI, V42, P3130
[5]   Tear levels of tumor necrosis factor-alpha in patients with Parkinson's disease [J].
Comoglu, Selim Selcuk ;
Guven, Hayat ;
Acar, Mutlu ;
Ozturk, Gulfer ;
Kocer, Bilge .
NEUROSCIENCE LETTERS, 2013, 553 :63-67
[6]   TEAR ANALYSIS IN MULTIPLE-SCLEROSIS [J].
COYLE, PK ;
SIBONY, PA .
NEUROLOGY, 1986, 36 (04) :547-550
[7]   OLIGOCLONAL IGG IN TEARS [J].
COYLE, PK ;
SIBONY, P ;
JOHNSON, C .
NEUROLOGY, 1987, 37 (05) :853-856
[8]  
COYLE PK, 1986, INVEST OPHTH VIS SCI, V27, P622
[9]   Post-collection processing of Schirmer strip-collected human tear fluid impacts protein content [J].
Denisin, Aleksandra K. ;
Karns, Kelly ;
Herr, Amy E. .
ANALYST, 2012, 137 (21) :5088-5096
[10]   Silver stained isoelectrophoresis of tears and cerebrospinal fluid in multiple sclerosis [J].
Devos, D ;
Forzy, G ;
de Seze, J ;
Caillez, S ;
Louchart, P ;
Gallois, P ;
Hautecoeur, P .
JOURNAL OF NEUROLOGY, 2001, 248 (08) :672-675