A rapid microplate method for quantifying inhibition of bacterial adhesion to eukaryotic cells

被引：20

作者：

Acord, J ^{[1
]}

Maskell, J ^{[1
]}

Sefton, A ^{[1
]}

机构：

[1] Barts & London Queen Marys Sch Med & Dent, Ctr Infect Dis, Inst Cell & Mol Sci, London E1 2AD, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2005年 / 60卷 / 01期

关键词：

microplate assay; bacterial adhesion; molecular mimicry; antiadhesive carbohydrates;

D O I：

10.1016/j.mimet.2004.08.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adhesion of bacteria to the mucosal epithelial cell surface is the first step in infection, and studies have shown that inhibition of this step may be useful therapeutically. To test compounds that may prevent bacterial binding to a number of epithelial cell lines, we have developed a high-throughput adhesion assay using a microtitre plate system and bacteria that have been modified to express firefly luciferase. This method has proved to be a sensitive, rapid, and reproducible system for screening antiadhesive agents for their effects on bacterial adhesion. 2004 Elsevier B.V. All rights reserved.

引用

页码：55 / 62

页数：8

共 23 条

[1] Novel bioluminescent assay of alkaline phosphatase using adenosine-3′-phosphate-5′-phosphosulfate as substrate and the luciferin-luciferase reaction and its application [J].

Arakawa, H ;

Shiokawa, M ;

Imamura, O ;

Maeda, M .

ANALYTICAL BIOCHEMISTRY, 2003, 314 (02) :206-211

[2] Inhibition by dextran of Pseudomonas aeruginosa adherence to epithelial cells [J].

Barghouthi, S ;

Guerdoud, LM ;

Speert, DP .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1996, 154 (06) :1788-1793

[3]

BARRANCO SC, 1983, CANCER RES, V43, P1703

[4]

CASJENS S, 1991, GENETICS, V127, P637

[5]

EDEN CS, 1978, SCAND J INFECT DIS, P5

[6] SALMONELLA INTERACTIONS WITH POLARIZED HUMAN INTESTINAL CACO-2 EPITHELIAL-CELLS [J].

FINLAY, BB ;

FALKOW, S .

JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (05) :1096-1106

[7] IN-VITRO CULTIVATION OF HUMAN TUMORS - ESTABLISHMENT OF CELL LINES DERIVED FROM A SERIES OF SOLID TUMORS [J].

GIARD, DJ ;

AARONSON, SA ;

TODARO, GJ ;

ARNSTEIN, P ;

KERSEY, JH ;

DOSIK, H ;

PARKS, WP .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1973, 51 (05) :1417-1423

[8] SELECTIVE BACTERIAL ADHERENCE TO ORAL EPITHELIAL SURFACES AND ITS ROLE AS AN ECOLOGICAL DETERMINANT [J].

GIBBONS, RJ ;

VANHOUTE, J .

INFECTION AND IMMUNITY, 1971, 3 (04) :567-+

[9]

HASHIMOTOGOTOH T, 1993, GENE, V137, P211

[10] 4 NEW DERIVATIVES OF THE BROAD-HOST-RANGE CLONING VECTOR PBBR1MCS, CARRYING DIFFERENT ANTIBIOTIC-RESISTANCE CASSETTES [J].

KOVACH, ME ;

ELZER, PH ;

HILL, DS ;

ROBERTSON, GT ;

FARRIS, MA ;

ROOP, RM ;

PETERSON, KM .

GENE, 1995, 166 (01) :175-176

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