Detection of antibodies to Kaposi's sarcoma-associated herpesvirus: A new approach using K8.1 ELISA and a newly developed recombinant LANA ELISA

被引：59

作者：

Mbisa, Georgina L. ^{[1
,2
]}

Miley, Wendell

Gamache, Christine J. ^{[1
,2
]}

Gillette, William K. ^{[3
]}

Esposito, Dominic ^{[3
]}

Hopkins, Ralph ^{[3
]}

Busch, Michael P. ^{[4
,5
]}

Schreiber, George B. ^{[6
]}

Little, Richard F. ^{[7
]}

Yarchoan, Robert ^{[7
]}

Ortiz-Conde, Betty A. ^{[1
,2
,3
]}

Labo, Nazzarena

Whitby, Denise

机构：

[1] NCI, Viral Oncol Sect, AIDS & Canc Virus Program, SAIC Frederick Inc, Frederick, MD 21702 USA

[2] NCI, Viral Technol Lab, Adv Technol Program, SAIC Frederick Inc, Frederick, MD 21702 USA

[3] NCI, Prot Express Lab, SAIC Frederick Inc, Frederick, MD 21702 USA

[4] Blood Syst Res Inst, San Francisco, CA 94118 USA

[5] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA

[6] Westat Corp, Rockville, MD 20850 USA

[7] NCI, HIV & AIDS Malignancy Branch, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2010年 / 356卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

KSHV serology; ELISA; LANA; K8.1; LATENT CLASS ANALYSIS; READING FRAME K8.1; NUCLEAR ANTIGEN; DNA-SEQUENCES; MONOCLONAL-ANTIBODIES; GENERAL-POPULATION; SEROLOGIC ASSAYS; HUMAN-HERPESVIRUS-8; INFECTION; IDENTIFICATION;

D O I：

10.1016/j.jim.2010.02.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Detection of antibodies to Kaposi's sarcoma-associated herpesvirus (KSHV or Human herpesvirus 8) is a topic of ongoing controversy. KSHV expresses multiple antigens and host responses are highly variable. We have previously described an algorithm for determining KSHV infection based on K8.1 ELISA and LANA immunofluorescence assay (IFA). Here we describe the development of a recombinant ELISA for LANA and an improved testing strategy using ELISAs for LANA and K8.1. We assessed mammalian and baculovirus expression systems for the production of full-length recombinant LANA. We evaluated the performance of LANA ELISAs using human serum samples from several sources including blood donors and clinical patients diagnosed with Kaposi's sarcoma and compared them to LANA IFA. Both LANA ELISAs exhibited comparable sensitivity and specificity to LANA IFA but showed considerably greater reliability. The LANA ELISA can thus be used in conjunction with the previously described K8.1 ELISA to enable the highly sensitive and specific detection of antibodies to KSHV. Use of this testing strategy will provide a more accurate and reliable diagnostic assessment of KSHV status. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：39 / 46

页数：8

共 28 条

[1] Antibody reactivity to latent and lytic antigens to human herpesvirus-8 in longitudinally followed homosexual men [J].