Optimization of cultivation medium for enhanced production of antifungal metabolites by Streptomyces hygroscopicus

被引：35

作者：

Grahovac, Jovana ^{[1
]}

Grahovac, Mila ^{[2
]}

Dodic, Jelena ^{[1
]}

Bajic, Bojana ^{[1
]}

Balaz, Jelica ^{[2
]}

机构：

[1] Univ Novi Sad, Fac Technol, Novi Sad 21000, Serbia

[2] Univ Novi Sad, Fac Agr, Novi Sad 21000, Serbia

来源：

CROP PROTECTION | 2014年 / 65卷

关键词：

Biological control; Optimization of medium components; Fermentation; Streptomyces hygroscopicus; Colletotrichum spp; Apples; BIOLOGICAL-CONTROL; RAW GLYCEROL; FERMENTATION; DISEASES;

D O I：

10.1016/j.cropro.2014.07.020

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Antifungal potential of Streptomyces hygroscopicus metabolites was tested against the isolates of Colletotrichum spp., obtained from rotten apple fruits. Experimental factorial design and response surface methodology were employed to optimize medium composition and enhance antifungal components productivity by S. hygroscopicus. Results of optimization suggest that the optimal medium composition is (g/L): glycerol (13.92), yeast extract (5.00), phosphates (0.63), CaCO3, (3.0), NaCl, (3.0), MgSO4, (0.5). Further research was performed to validate the obtained results and confirm their applicability in planta on artificially inoculated apple fruits. The obtained efficacy after 14 days of incubation was 99.3% against Colletotrichum acutatum and 99.8% against Colletotrichum gloeosporioides. Application of supernatant of S. hygroscopicus cultivation medium, produced under defined conditions, in biological control of apple rot could offer safe and healthy apple fruits on the market, while simultaneously helping to solve the problem of waste glycerol disposal in the biodiesel industry. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：143 / 152

页数：10

共 35 条

[1] Studies of actinomycetes for biological control of Colletotrichum musae pathogen during post harvest anthracnose of banana [J].