Cholesterol oxidases act as signaling proteins for the biosynthesis of the polyene macrolide pimaricin

被引:62
作者
Mendes, Marta V.
Recio, Eliseo
Anton, Nuria
Guerra, Susana M.
Santos-Aberturas, Javier
Martin, Juan F.
Aparicio, Jesus F. [1 ]
机构
[1] INBIOTEC, Inst Biotecnol, Leon 24006, Spain
[2] Univ Leon, Fac Biol, Area Microbiol, E-24071 Leon, Spain
来源
CHEMISTRY & BIOLOGY | 2007年 / 14卷 / 03期
关键词
D O I
10.1016/j.chembiol.2007.01.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The gene cluster responsible for pimaricin biosynthesis in Streptomyces natalensis contains a cholesterol oxidase-encoding gene (pimE) surrounded by genes involved in pimaricin production. Gene-inactivation and -complementation experiments revealed that pimE encodes a functional cholesterol oxidase and, surprisingly, that it is also involved in pimaricin biosynthesis. This extracellular enzyme was purified from S. natalensis culture broths to homogeneity, and it was shown to restore pimaricin production when added to the mutant culture broths. Other cholesterol oxidases also triggered pimaricin production, suggesting that these enzymes could act as signaling proteins for polyene biosynthesis. This finding constitutes the description of a cholesterol oxidase gene with an involvement in antibiotic biosynthesis, and it broadens the scope of the biological functions for this type of oxidase.
引用
收藏
页码:279 / 290
页数:12
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