Determination of phenolic acids in olive oil by capillary electrophoresis

被引:18
作者
Buiarelli, F
Di Berardino, S
Coccioli, F
Jasionowska, R
Russo, MV
机构
[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy
[2] Univ Molise, Fac Agr DISTAAM, I-86100 Campobasso, Italy
关键词
D O I
10.1002/adic.200490087
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A CZE method for the separation and quantitation of phenolic acids (cinnamic, syringic, p-coumaric, vanillic, caffeic, 3,4-dihydroxyphenylacetic, protocatechuic), extracted from extra virgin olive oil, was developed. The sample preparation involved the LLE and SPE extraction methods. CE separation was performed in a fused silica capillary of I.D. = 50mum using as a BGE 40 mM borate buffer at pH=9.2. The separation voltage was 18kV with corresponding current of 27-28 muA. Detection was accomplished with UV-detector at lambda=200nm. The proposed method was fully validated. A good repeatability of migration time (RSD% ranged from 0.81 to 1.63) and of corrected peak area ( RSD% from 2.89 to 5.77) was obtained. The linearity of detector response in the range from 5 to 50 ppm was checked, obtaining the correlation coefficient R 2 values in the range: 0.9919-0.9997. Some phenolic acids in real oil samples were detected and quantified with the proposed method.
引用
收藏
页码:699 / 705
页数:7
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