Maturation State and Function of Monocyte Derived Dendritic Cells in Liver Transplant Recipients

被引:0
作者
Shariat, Afsoon [1 ]
Karimi, Mohammad Hossein [2 ]
Mokhtariazad, Talat [3 ]
Moazzeni, Seyed Mohammad [4 ]
Geramizadeh, Bita [2 ]
MalekHosseini, Seyed Ali [5 ]
Yaghobi, Ramin [2 ]
机构
[1] Islamic Azad Univ, Tehran Sci & Res Branch, Dept Biol, Coll Basic Sci, Tehran, Iran
[2] Shiraz Univ Med Sci, Namazi Hosp, Shiraz Transplant Res Ctr, Shiraz, Iran
[3] Univ Tehran Med Sci, Sch Publ Hlth, Dept Virol, Tehran, Iran
[4] Tarbiat Modares Univ, Dept Immunol, Fac Med Sci, Tehran, Iran
[5] Shiraz Univ Med Sci, Namazi Hosp, Shiraz Transplant Ctr, Shiraz, Iran
基金
美国国家科学基金会;
关键词
Dendritic Cells; Liver Transplantation; TLR; ACUTE ALLOGRAFT-REJECTION; INTERLEUKIN-12; FAMILY; IL-12; IMMUNOTHERAPY; RECOGNITION; SIGNALS; CD83;
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: Dendritic cells (DCs) are potent antigen presenting cells for triggering of the immune reaction post transplantation. These cells are centrally involved in the initiation of T cell-dependent immune responses. Objective: To compare the level of DC maturation and function in liver transplant recipients with healthy controls. Methods: In this study, twelve peripheral blood samples were selected from six liver transplant patients and six healthy controls. After the generation of DCs from monocytes, expression levels and mean fluorescent intensity (MFI) of several DC maturation markers were evaluated using flowcytometry. Secretion of IL-6, IL-12 and IL-23 proinflammatory cytokines was determined using ELISA. Gene expressions of TLR-2, TLR-4 and IL-23 were analyzed using real-time PCR. Results: DC expression markers including CD83 (p=0.007) and CD86 (p=0.02), as well as secretion of IL-6 (p=0.02) and IL-12 (p=0.007) by DCs were significantly increased in liver transplant patients compared with healthy controls. The MFI of CD86 (p=0.009) and HLA-DR (p=0.005) expression on DCs was also higher in patients. The expression of TLR-2 transcripts in DCs of patients was higher than that of the controls (p=0.03). Conclusion: Based on these findings, increased frequency of DCs expressing CD83 and CD86, higher expression of CD86, HLA-DR, and TLR-2 as well as elevated secretion of proinflammatory cytokines in DCs of liver transplant recipient's point to the more mature phenotype and active function of DCs in patients compared with controls.
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收藏
页码:153 / 165
页数:13
相关论文
共 35 条
  • [1] Interleukins 1β and 6 but not transforming growth factor-β are essential for the differentiation of interleukin 17-producing human T helper cells
    Acosta-Rodriguez, Eva V.
    Napolitani, Giorgio
    Lanzavecchia, Antonio
    Sallusto, Federica
    [J]. NATURE IMMUNOLOGY, 2007, 8 (09) : 942 - 949
  • [2] Migration of Allosensitizing Donor Myeloid Dendritic Cells into Recipients After Liver Transplantation
    Bosma, Brenda M.
    Metselaar, Herold J.
    Gerrits, Jeroen H.
    van Besouw, Nicole M.
    Mancham, Shanta
    Groothuismink, Zwier M. A.
    Boor, Patrick P. C.
    van der Laan, Luc J. W.
    Tilanus, Hugo W.
    Kuipers, Ernst J.
    Kwekkeboom, Jaap
    [J]. LIVER TRANSPLANTATION, 2010, 16 (01) : 12 - 22
  • [3] Anti-IL-23 antibody blockade of IL-23/IL-17 pathway attenuates airway obliteration in rat orthotopic tracheal transplantation
    Cao, Hao
    Lan, Qin
    Shi, Qian
    Zhou, Xiaohui
    Liu, Gang
    Liu, Jian
    Tang, Guangliang
    Qiu, Chenli
    Qiu, Chao
    Xu, Jianqing
    Fan, Huimin
    Liu, Zhongmin
    [J]. INTERNATIONAL IMMUNOPHARMACOLOGY, 2011, 11 (05) : 569 - 575
  • [4] TLR Signals Promote IL-6/IL-17-Dependent Transplant Rejection
    Chen, Luqiu
    Ahmed, Emily
    Wang, Tongmin
    Wang, Ying
    Ochando, Jordi
    Chong, Anita S.
    Alegre, Maria-Luisa
    [J]. JOURNAL OF IMMUNOLOGY, 2009, 182 (10) : 6217 - 6225
  • [5] Transplantation immunology: Solid organ and bone marrow
    Chinen, Javier
    Buckley, Rebecca H.
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2010, 125 (02) : S324 - S335
  • [6] The role of toll-like receptors 2 and 4 in acute allograft rejection after liver transplantation
    Deng, J. -F.
    Geng, L.
    Qian, Y. -G.
    Li, H.
    Wang, Y.
    Xie, H. -Y.
    Feng, X. -W.
    Zheng, S. -S.
    [J]. TRANSPLANTATION PROCEEDINGS, 2007, 39 (10) : 3222 - 3224
  • [7] Human monocyte isolation methods influence cytokine production from in vitro generated dendritic cells
    Elkord, E
    Williams, PE
    Kynaston, H
    Rowbottom, AW
    [J]. IMMUNOLOGY, 2005, 114 (02) : 204 - 212
  • [8] Generating potent Th1/Tc1 T cell adoptive immunotherapy doses using human IL-12: Harnessing the immunomodulatory potential of IL-12 without the in vivo-associated toxicity
    Emtage, PCR
    Clarke, D
    Gonzalo-Dagonzo, R
    Junghans, RP
    [J]. JOURNAL OF IMMUNOTHERAPY, 2003, 26 (02): : 97 - 106
  • [9] Changes in the Serum Levels of Interleukin-17/Interleukin-23 During Acute Rejection in Liver Transplantation
    Fabrega, Emilio
    Lopez-Hoyos, Marcos
    Segundo, David San
    Casafont, Fernandoo
    Pons-Romero, Fernando
    [J]. LIVER TRANSPLANTATION, 2009, 15 (06) : 629 - 633
  • [10] CD4 T cell-dependent conditioning of dendritic cells to produce IL-12 results in CD8-mediated graft rejection and avoidance of tolerance
    Filatenkov, AA
    Jacovetty, EL
    Fischer, UB
    Curtsinger, JM
    Mescher, MF
    Ingulli, E
    [J]. JOURNAL OF IMMUNOLOGY, 2005, 174 (11) : 6909 - 6917