Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

被引：11

作者：

Choi, Eun Sik ^{[1
,2
,3
]}

Lee, Yun Jung ^{[1
,2
,3
]}

Seo, Chang Seob ^{[4
]}

Yoon, Jung Joo ^{[1
,2
,3
]}

Han, Byung Hyuk ^{[1
,2
,3
]}

Park, Min Cheol ^{[5
]}

Kang, Dae Gill ^{[1
,2
,3
]}

Lee, Ho Sub ^{[1
,2
,3
]}

机构：

[1] Wonkwang Univ, Coll Oriental Med, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea

[2] Wonkwang Univ, Profess Grad Sch Oriental Med, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea

[3] Wonkwang Univ, Hanbang Body Fluid Res Ctr, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea

[4] Korea Inst Oriental Med, K Herb Res Ctr, 1672 Yuseong Daero, Daejeon 34054, South Korea

[5] Wonkwang Univ, Coll Oriental Med, Dept Oriental Med Ophthalmol & Otolaryngol & Derm, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2016年 / 2016卷

基金：

新加坡国家研究基金会;

关键词：

NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; SI-WU-TANG; HEME OXYGENASE-1; ENDOTHELIAL-CELLS; RAT LIVERS; CONSTITUENTS; ACTIVATION; EXPRESSION; MEDICINE;

D O I：

10.1155/2016/9580234

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Samul-Tang (Si-Wu-Tang, SMT), composed of four medicinal herbs, is a well-known herbal formula treating hematological disorder or gynecologic disease. However, vascular protective effects of SMT and its molecular mechanisms on the vascular endothelium, known as the central spot of vascular inflammatory process, are not reported. The aim of this study was to investigate vascular protective effects of SMT water extract in human umbilical vein endothelial cells (HUVECs). Water extract of SMT was prepared and identified by HPLC-PDA analysis. Expression of cell adhesion molecules (CAMs) and heme oxygenase-1 (HO-1) and translocation of nuclear factor-kappa B (NF-kappa B) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined by western blot. Nuclear localization of NF-kappa B and Nrf2 was visualized by immunofluorescence and DNA binding activity of NF-kappa B was measured. ROS production, HL-60 monocyte adhesion, and intracellular nitric oxide (NO) were also measured using a fluorescent indicator. SMT suppressed NF-kappa B translocation and activation as well as expression of CAMs, monocyte adhesion, and ROS production induced by TNF-alpha in HUVECs. SMT treated HUVECs showed upregulation of HO-1 and NO which are responsible for vascular protective action. Our study suggests that SMT, a traditionally used herbal formula, protects the vascular endothelium from inflammation and might be used as a promising vascular protective drug.

引用

页数：14

共 44 条

[1] Phenotypic heterogeneity of the endothelium I. Structure, function, and mechanisms [J].