NtcA from Microcystis aeruginosa PCC 7806 Is Autoregulatory and Binds to the Microcystin Promoter

被引：79

作者：

Ginn, H. P. ^{[1
]}

Pearson, L. A. ^{[1
]}

Neilan, B. A. ^{[1
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 13期

基金：

澳大利亚研究理事会;

关键词：

TRANSCRIPTION FACTOR NTCA; GLOBAL NITROGEN REGULATOR; SP STRAIN PCC-7942; CYANOBACTERIUM SYNECHOCOCCUS; GENE-CLUSTER; CHLAMYDOMONAS-REINHARDTII; ANABAENA PCC-7120; IN-VITRO; EXPRESSION; SYNTHETASE;

D O I：

10.1128/AEM.01862-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

NtcA is a transcription factor that has been found in a diverse range of cyanobacteria. This nitrogen-controlled factor was focused on as a key component in the yet-to-be-deciphered regulatory network controlling microcystin production. Adaptor-mediated PCR was utilized to isolate the ntcA gene from Microcystis aeruginosa PCC 7806. This gene was cloned, and the recombinant (His-tagged) protein was overexpressed and purified for use in mobility shift assays to analyze NtcA binding to putative sites identified in the microcystin mcyA/D promoter region. Autoregulation of NtcA in M. aeruginosa was shown via NtcA binding in the upstream ntcA promoter region. The observation of binding of NtcA to the mcyA/D promoter region has direct relevance for the regulation of microcystin biosynthesis, as transcription of the mcyABCDEFGHIJ gene cluster appears to be under direct control of nitrogen.

引用

页码：4362 / 4368

页数：7

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