NtcA from Microcystis aeruginosa PCC 7806 Is Autoregulatory and Binds to the Microcystin Promoter

被引：79

作者：

Ginn, H. P. ^{[1
]}

Pearson, L. A. ^{[1
]}

Neilan, B. A. ^{[1
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 13期

基金：

澳大利亚研究理事会;

关键词：

TRANSCRIPTION FACTOR NTCA; GLOBAL NITROGEN REGULATOR; SP STRAIN PCC-7942; CYANOBACTERIUM SYNECHOCOCCUS; GENE-CLUSTER; CHLAMYDOMONAS-REINHARDTII; ANABAENA PCC-7120; IN-VITRO; EXPRESSION; SYNTHETASE;

D O I：

10.1128/AEM.01862-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

NtcA is a transcription factor that has been found in a diverse range of cyanobacteria. This nitrogen-controlled factor was focused on as a key component in the yet-to-be-deciphered regulatory network controlling microcystin production. Adaptor-mediated PCR was utilized to isolate the ntcA gene from Microcystis aeruginosa PCC 7806. This gene was cloned, and the recombinant (His-tagged) protein was overexpressed and purified for use in mobility shift assays to analyze NtcA binding to putative sites identified in the microcystin mcyA/D promoter region. Autoregulation of NtcA in M. aeruginosa was shown via NtcA binding in the upstream ntcA promoter region. The observation of binding of NtcA to the mcyA/D promoter region has direct relevance for the regulation of microcystin biosynthesis, as transcription of the mcyABCDEFGHIJ gene cluster appears to be under direct control of nitrogen.

引用

页码：4362 / 4368

页数：7

共 34 条

[1] Marine cyanobacteria - a prolific source of natural products [J].

Burja, AM ;

Banaigs, B ;

Abou-Mansour, E ;

Burgess, JG ;

Wright, PC .

TETRAHEDRON, 2001, 57 (46) :9347-9377

[2] CYANOBACTERIA SECONDARY METABOLITES - THE CYANOTOXINS [J].

CARMICHAEL, WW .

JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (06) :445-459

[3] Altered expression of two light-dependent genes in a microcystin-lacking mutant of Microcystis aeruginosa PCC 7806 [J].

Dittmann, E ;

Erhard, M ;

Kaebernick, M ;

Scheler, C ;

Neilan, BA ;

von Döhren, H ;

Börner, T .

MICROBIOLOGY-SGM, 2001, 147 :3113-3119

[4]

FRIAS JE, 1993, J BACTERIOL, V175, P5710

[5] Nitrogen control in cyanobacteria [J].

Herrero, A ;

Muro-Pastor, AM ;

Flores, E .

JOURNAL OF BACTERIOLOGY, 2001, 183 (02) :411-425

[6] Evidence for redox regulation of the transcription factor NtcA, acting both as an activator and a repressor, in the cyanobacterium Anabaena PCC 7120 [J].

Jiang, F ;

Mannervik, B ;

Bergman, B .

BIOCHEMICAL JOURNAL, 1997, 327 :513-517

[7] CLONING, SEQUENCING, AND REGULATION OF THE GLUTATHIONE-REDUCTASE GENE FROM THE CYANOBACTERIUM ANABAENA PCC-7120 [J].

JIANG, FY ;

HELLMAN, U ;

SROGA, GE ;

BERGMAN, B ;

MANNERVIK, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (39) :22882-22889

[8] Examination of the transcription factor NtcA-binding motif by in vitro selection of DNA sequences from a random library [J].

Jiang, FY ;

Wisén, S ;

Widersten, M ;

Bergman, B ;

Mannervik, B .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 301 (04) :783-793

[9] Light and the transcriptional response of the microcystin biosynthesis gene cluster [J].

Kaebernick, M ;

Neilan, BA ;

Börner, T ;

Dittmann, E .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) :3387-3392

[10] Regulation of ntcA expression and nitrite uptake in the marine Synechococcus sp. strain WH 7803 [J].

Lindell, D ;

Padan, E ;

Post, AF .

JOURNAL OF BACTERIOLOGY, 1998, 180 (07) :1878-1886

← 1 2 3 4 →