Tumor-targeting Salmonella typhimurium A1-R arrests a doxorubicin-resistant PDGFRA-amplified patient-derived orthotopic xenograft mouse model of pleomorphic liposarcoma

被引：13

作者：

Kiyuna, Tasuku ^{[1
,2
,3
]}

Tome, Yasunori ^{[3
]}

Murakami, Takashi ^{[1
,2
]}

Zhao, Ming ^{[1
]}

Miyake, Kentaro ^{[1
,2
]}

Igarashi, Kentaro ^{[1
,2
]}

Kawaguchi, Kei ^{[1
,2
]}

Miyake, Masuyo ^{[1
,2
]}

Oshiro, Hiromichi ^{[1
,2
]}

Higuchi, Takashi ^{[1
,2
]}

Li, Yunfeng ^{[4
]}

Dry, Sarah M. ^{[4
]}

Nelson, Scott D. ^{[4
]}

Russell, Tara A. ^{[5
]}

Eckardt, Mark A. ^{[6
]}

Singh, Arun S. ^{[7
]}

Kanaya, Fuminori ^{[3
]}

Eilber, Fritz C. ^{[5
]}

Hoffman, Robert M. ^{[1
,2
]}

机构：

[1] AntiCancer Inc, 7917 Ostrow St, San Diego, CA 92111 USA

[2] Univ Calif San Diego, Dept Surg, San Diego, CA 92103 USA

[3] Univ Ryukyus, Grad Sch Med, Dept Orthoped Surg, 207 Uehara, Nishihara, Okinawa 9030215, Japan

[4] Univ Calif Los Angeles, Dept Pathol, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, Div Surg Oncol, 10833 LeConte Ave,Rm 54-140 CHS, Los Angeles, CA 90095 USA

[6] Yale Sch Med, Dept Surg, New Haven, CT USA

[7] Univ Calif Los Angeles, Div Hematol Oncol, Los Angeles, CA 90095 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2018年 / 119卷 / 09期

关键词：

green fluorescent protein; patient-derived orthotopic xenograft; PDGFRA amplification; pleomorphic liposarcoma; Salmonella typhimurium A1-R; soft-tissue sarcoma; tumor targeting; SUBCUTANEOUS ECTOPIC MODEL; HUMAN PANCREATIC-CANCER; SOFT-TISSUE SARCOMA; HUMAN COLON-CANCER; ANTI-CEA ANTIBODY; FUS-ERG FUSION; EWINGS-SARCOMA; PDOX MODEL; NUDE-MICE; COMBINATION;

D O I：

10.1002/jcb.27183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pleomorphic liposarcoma (PLPS) is a recalcitrant soft-tissue sarcoma (STS) subtype in need of transformative therapy. We have previously established a patient-derived orthotopic xenograft (PDOX) model, of PLPS with PDGFRA amplification, using surgical orthotopic implantation. In the current study, the PLPS PDOX model was randomized into 3 groups of 7 mice each: untreated control; doxorubicin (DOX)-treated; and treated with Salmonella typhimurium A1-R (S. typhimurium A1-R) expressing green fluorescent protein (GFP). Tumor volume and body weight were monitored during the treatment period. The PLPS PDOX was resistant to DOX. In contrast, the PLPS PDOX was highly sensitive to S. typhimurium A1-R. There was no significant body-weight loss among these 3 groups. Fluorescence imaging demonstrated that S. typhimurium A1-R-GFP was very effective to target the PLPS PDOX tumor. The current study demonstrates that a PLPS PDOX, resistant to first-line therapy DOX, was highly sensitive to tumor targeting S. typhimurium A1-R.

引用

页码：7827 / 7833

页数：7

共 41 条

[21] Intra-arterial administration of tumor-targeting Salmonella typhimurium A1-R regresses a cisplatin-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model [J].

Igarashi, Kentaro ;

Kawaguchi, Kei ;

Murakami, Takashi ;

Kiyuna, Tasuku ;

Miyake, Kentaro ;

Nelson, Scott D. ;

Dry, Sarah M. ;

Li, Yunfeng ;

Yanagawa, Jane ;

Russell, Tara A. ;

Singh, Arun S. ;

Yamamoto, Norio ;

Hayashi, Katsuhiro ;

Kimura, Hiroaki ;

Miwa, Shinji ;

Tsuchiya, Hiroyuki ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

CELL CYCLE, 2017, 16 (12) :1164-1170

[22] Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model [J].

Igarashi, Kentaro ;

Kawaguchi, Kei ;

Kiyuna, Tasuku ;

Murakami, Takashi ;

Miwa, Shinji ;

Nelson, Scott D. ;

Dry, Sarah M. ;

Li, Yunfeng ;

Singh, Arun ;

Kimura, Hiroaki ;

Hayashi, Katsuhiro ;

Yamamoto, Norio ;

Tsuchiya, Hiroyuki ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

CELL CYCLE, 2017, 16 (01) :91-94

[23] Combination treatment with recombinant methioninase enables temozolomide to arrest a BRAF V600E melanoma in a patient-derived orthotopic xenograft (PDOX) mouse model [J].

Kawaguchi, Kei ;

Igarashi, Kentaro ;

Li, Shukuan ;

Han, Qinghong ;

Tan, Yuying ;

Kiyuna, Tasuku ;

Miyake, Kentaro ;

Murakami, Takashi ;

Chmielowski, Bartosz ;

Nelson, Scott D. ;

Russell, Tara A. ;

Dry, Sarah M. ;

Li, Yunfeng ;

Unno, Michiaki ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

ONCOTARGET, 2017, 8 (49) :85516-85525

[24] Salmonella typhimurium A1-R targeting of a chemotherapy-resistant BRAF-V600E melanoma in a patient-derived orthotopic xenograft (PDOX) model is enhanced in combination with either vemurafenib or temozolomide [J].

Kawaguchi, Kei ;

Igarashi, Kentaro ;

Murakami, Takashi ;

Kiyuna, Tasuku ;

Zhao, Ming ;

Zhang, Yong ;

Nelson, Scott D. ;

Russell, Tara A. ;

Dry, Sarah M. ;

Singh, Arun S. ;

Chmielowski, Bartosz ;

Li, Yunfeng ;

Unno, Michiaki ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

CELL CYCLE, 2017, 16 (13) :1288-1294

[25] MEK inhibitors cobimetinib and trametinib, regressed a gemcitabine-resistant pancreatic-cancer patient-derived orthotopic xenograft (PDOX) [J].

Kawaguchi, Kei ;

Igarashi, Kentaro ;

Murakami, Takashi ;

Kiyuna, Tasuku ;

Lwin, Thinzar M. ;

Hwang, Ho Kyoung ;

Delong, Jonathan C. ;

Clary, Bryan M. ;

Bouvet, Michael ;

Unno, Michiaki ;

Hoffman, Robert M. .

ONCOTARGET, 2017, 8 (29) :47490-47496

[26] Combination of gemcitabine and docetaxel regresses both gastric leiomyosarcoma proliferation and invasion in an imageable patient-derived orthotopic xenograft (iPDOX) model [J].

Kawaguchi, Kei ;

Igarashi, Kentaro ;

Murakami, Takashi ;

Kiyuna, Tasuku ;

Nelson, Scott D. ;

Dry, Sarah M. ;

Li, Yunfeng ;

Russell, Tara A. ;

Singh, Arun S. ;

Chmielowski, Bartosz ;

Unno, Michiaki ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

CELL CYCLE, 2017, 16 (11) :1063-1069

[27] Tumor-Targeting Salmonella typhimurium A1-R Sensitizes Melanoma With a BRAF-V600E Mutation to Vemurafenib in a Patient-Derived Orthotopic Xenograft (PDOX) Nude Mouse Model [J].

Kawaguchi, Kei ;

Igarashi, Kentaro ;

Murakami, Takashi ;

Zhao, Ming ;

Zhang, Yong ;

Chmielowski, Bartosz ;

Kiyuna, Tasuku ;

Nelson, Scott D. ;

Russell, Tara A. ;

Dry, Sarah M. ;

Li, Yunfeng ;

Unno, Michiaki ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2017, 118 (08) :2314-2319

[28] Tumor-targeting Salmonella typhimurium A1-R combined with temozolomide regresses malignant melanoma with a BRAF-V600E mutation in a patient-derived orthotopic xenograft (PDOX) model [J].

Kawaguchi, Kei ;

Igarashi, Kentaro ;

Murakami, Takashi ;

Chmielowski, Bartosz ;

Kiyuna, Tasuku ;

Zhao, Ming ;

Zhang, Yong ;

Singh, Arun ;

Unno, Michiaki ;

Nelson, Scott D. ;

Russell, Tara A. ;

Dry, Sarah M. ;

Li, Yunfeng ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

ONCOTARGET, 2016, 7 (52) :85929-85936

[29] Vemurafenib-resistant BRAF-V600E-mutated melanoma is regressed by MEK-targeting drug trametinib, but not cobimetinib in a patient-derived orthotopic xenograft (PDOX) mouse model [J].

Kawaguchi, Kei ;

Murakami, Takashi ;

Chmielowski, Bartosz ;

Igarashi, Kentaro ;

Kiyuna, Tasuku ;

Unno, Michiaki ;

Nelson, Scott D. ;

Russell, Tara A. ;

Dry, Sarah M. ;

Li, Yunfeng ;

Eilber, Fritz C. ;

Hoffman, Robert M. .

ONCOTARGET, 2016, 7 (44) :71737-71743

[30]

Kiyuna T, TISSUE CELL

← 1 2 3 4 5 →