Threshold in Stage-specific Embryonic Glycotypes Uncovered by a Full Portrait of Dynamic N-Glycan Expression during Cell Differentiation

被引：46

作者：

Amano, Maho ^{[1
,2
]}

Yamaguchi, Misa ^{[1
,2
]}

Takegawa, Yasuhiro ^{[1
,2
]}

Yamashita, Tadashi ^{[1
,2
]}

Terashima, Michiyo ^{[3
]}

Furukawa, Jun-ichi ^{[1
,2
]}

Miura, Yoshiaki ^{[4
]}

Shinohara, Yasuro ^{[1
,2
]}

Iwasaki, Norimasa ^{[3
]}

Minami, Akio ^{[3
]}

Nishimura, Shin-ichiro ^{[1
,2
,4
]}

机构：

[1] Hokkaido Univ, Lab Adv Chem Biol, Grad Sch Life Sci, Kita Ku, Sapporo, Hokkaido 0010021, Japan

[2] Hokkaido Univ, Frontier Res Ctr Postgenome Sci & Technol, Kita Ku, Sapporo, Hokkaido 0010021, Japan

[3] Hokkaido Univ, Sch Med, Dept Orthoped Surg, Sapporo, Hokkaido 0608638, Japan

[4] Ezose Sci Inc, Pine Brook, NJ 07058 USA

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2010年 / 9卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

NEURONAL DIFFERENTIATION; PROTEOMIC ANALYSIS; STEM-CELLS; GLYCOSYLATION; MUSCLE; OLIGOSACCHARIDES; GLYCOPROTEIN;

D O I：

10.1074/mcp.M900559-MCP200

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although various glycoforms appear to participate independently in multiple molecular interactions in cellular adhesion that contribute to embryogenesis and organogenesis, a full portrait of the glycome diversity and the effect of the structural variations of cellular glycoforms on individual cell stages in proliferation and differentiation remain unclear. Here we describe a novel concept for the characterization of dynamic glycoform alteration during cell differentiation by means of "glycoblotting-based cellular glycomics," the only method allowing for rapid and quantitative glycan analysis. We demonstrated that processes of dynamic cellular differentiation of mouse embryonic carcinoma cells, P19CL6 and P19C6, and mouse embryonic stem cells into cardiomyocytes or neural cells can be monitored and characterized quantitatively by profiling entire N-glycan structures of total cell glycoproteins. Whole N-glycans enriched and identified by the glycoblotting method (67 glycans for P19CL6, 75 glycans for P19C6, and 72 glycans for embryonic stem cells) were profiled and bar-coded quantitatively with respect to the ratio of subgroups composed of characteristic glycoforms, namely glycotypes. Molecular & Cellular Proteomics 9: 523-537, 2010.

引用

页码：523 / 537

页数：15

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