Anticancer and antibacterial potential of MDR Staphylococcus aureus mediated synthesized silver nanoparticles

The increasing cases of resistance, low efficacy and high toxicity of antibiotics and anticancer agents have led to the discovery of nanoparticles as potent antimicrobial and anticancer agents to combat the threat of Multi Drug Resistance (MDR) and to minimize the side effects of drugs. The synthesized silver nanoparticles (SAgNPs) (synthesized using MDR Staphylococcus aureus) were characterized to confirm synthesis, shape, size, hydrodynamic diameter, colloidal stability and surface functionalization, by UV-visible spectroscopy, TEM, DLS, zeta potential test and FTIR respectively. SAgNPs were found to have spherical shape with a size of 15 nm. Hydrodynamic diameter of nanoparticles was found to be 88.65 nm and the value of zeta potential was recorded to be -24.03 mV. The antimicrobial and anticancer potential of SAgNPs was performed against normal & MDR strains of S. aureus and human colon cancer cell line (HCT-116), respectively.. The MICs of SAgNPs against normal and MDR S. aureus strains were found to be 0.025 mu g/ml & 0.053 mu g/ml, respectively. Similarly, IC50 against HCT-116 cell line was found to be 0.069 mu g/ml by MTT Assay. DAPI analysis confirmed the interaction of SAgNPs with DNA in order to initiate apoptosis for killing cancer cells.