Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay

被引：12

作者：

Ge, Jinying

Wen, Zhiyun

Wang, Xiyun

Hu, Sen

Liu, Yonggang

Kong, Xiangang

Chen, Hualan

Bu, Zhigao

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

[2] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

来源：

IMPACT OF EMERGING ZOONOTIC DISEASES ON ANIMAL HEALTH | 2006年 / 1081卷

关键词：

SARS coronavirus; spike protein; VSV; pseudotype;

D O I：

10.1196/annals.1373.030

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Delta G* SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Delta G*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSV Delta G*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.

引用

页码：246 / 248

页数：3

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