Anti-oxidative stress role of valsartan and fluvastatin in rat mesangial cells cultured with high glucose

This study aims to investigate the roles of valsartan and fluvastatin in fighting against oxidative stress injury in high glucose cultured rat mesangial cells (HBZY-1). The rat mesangial cells were cultured in vitro, then divided into the control group, high glucose group, mannitol group, high glucose plus valsartan group, high glucose plus fluvastatin group, and high glucose plus valsartan and fluvastatin group. Indicators such as the cell morphology, cell viability, superoxide dismutase (SOD), nitric oxide (NO) and malondialdehyde (MDA) were compared. Compared with the control group, the high glucose culture could significantly reduce the SOD activity (61.54 +/- 0.30 vs 30.99 +/- 0.47, P<0.05) and the NO level (9.56 +/- 0.43 vs 17.11 +/- 0.24, P<0.05), while significantly increase the MDA concentration (36.2 +/- 0.13 vs 10.21 +/- 0.14, P<0.05) in the supernatant of rat mesangial cells. While the culture of high glucose with different concentrations of valsartan and fluvastatin could increase the SOD activity and NO level, and reduce the MDA concentration, among which the high glucose + valsartan + fluvastatin culture group exhibtied the most significant effects. Valsartan and fluvastatin exhibited the dose-dependent effects of anti-oxidative stress injury in high glucose cultured rat mesangial cells, and the combination of these two drugs had better effects than single drug.