Structural and Functional Properties of the C-terminal Region of Mitochondrial ADP/ATP Carrier

被引：1

作者：

Shinohara, Yasuo ^{[1
,3
]}

Hashimoto, Mitsuru ^{[4
]}

Kihira, Yoshitaka ^{[2
]}

Ohkura, Kazuto ^{[5
]}

Majima, Eiji ^{[6
]}

Terada, Hiroshi ^{[7
]}

机构：

[1] Univ Tokushima, Inst Genome Res, Tokushima 7708503, Japan

[2] Univ Tokushima, Inst Hlth Biosci, Tokushima 7708503, Japan

[3] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan

[4] Matsuyama Univ, Fac Pharmaceut Sci, Matsuyama, Ehime 7908578, Japan

[5] Chiba Inst Sci, Fac Pharm, Choshi 2880025, Japan

[6] Protenova Inc, Takamatsu, Kagawa 7610301, Japan

[7] Tokyo Univ Sci, Fac Pharmaceut Sci, Noda, Chiba 2788510, Japan

来源：

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN | 2010年 / 130卷 / 02期

关键词：

ADP/ATP carrier (AAC); solute carrier family; mitochondria; transport mechanism; yeast; SITE-DIRECTED MUTAGENESIS; YEAST; EXPRESSION; ADP;

D O I：

10.1248/yakushi.130.199

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Mitochondrial ADP/ATP carrier (AAC) is a protein catalyzing the transport of adenine nucleotides across inner mitochondrial membrane. In this review article, we first briefly introduce structural and functional properties of this protein. Next, we describe the results of our recent studies on the difference in the C-terminal region between yeast type 2 AAC isoform and bovine type 1 AAC isoform. Furthermore, based on the reactivities of cysteine residues that replaced amino acids in the sixth transmembrane segment, the probable structural features of the C-terminal region of this carrier are discussed.

引用

页码：199 / 204

页数：6

共 15 条

[1] A fourth ADP/ATP carrier isoform in man: identification, bacterial expression, functional characterization and tissue distribution [J].

Dolce, V ;

Scarcia, P ;

Iacopetta, D ;

Palmieri, F .

FEBS LETTERS, 2005, 579 (03) :633-637

[2] The mitochondrial ADP/ATP carrier:: Structural, physiological and pathological aspects [J].

Fiore, C ;

Trézéguet, V ;

Le Saux, A ;

Roux, P ;

Schwimmer, C ;

Dianoux, AC ;

Noel, F ;

Lauquin, GJM ;

Brandolin, G ;

Vignais, PV .

BIOCHIMIE, 1998, 80 (02) :137-150

[3] Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers [J].

Hashimoto, M ;

Shinohara, Y ;

Majima, E ;

Hatanaka, T ;

Yamazaki, N ;

Terada, H .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1999, 1409 (03) :113-124

[4] Characterization of loops of the yeast mitochondrial ADP/ATP carrier facing the cytosol by site-directed mutagenesis [J].

Hatanaka, T ;

Kihira, Y ;

Shinohara, Y ;

Majima, E ;

Terada, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 286 (05) :936-942

[5] Functionally important conserved length of C-terminal regions of yeast and bovine ADP/ATP carriers, identified by deletion mutants studies, and water accessibility of the amino acids at the C-terminal region of the yeast carrier [J].

Iwahashi, Akihiro ;

Ishii, Aoi ;

Yamazaki, Naoshi ;

Hashimoto, Mitsuru ;

Ohkura, Kazuto ;

Kataoka, Masatoshi ;

Majima, Eiji ;

Terada, Hiroshi ;

Shinohara, Yasuo .

MITOCHONDRION, 2008, 8 (02) :196-204

[6] Cysteine labeling studies detect conformational changes in region 106-132 of the mitochondrial ADP/ATP carrier of Saccharomyces cerevisiae [J].

Kihira, Y ;

Majima, E ;

Shinohara, Y ;

Terada, H .

BIOCHEMISTRY, 2005, 44 (01) :184-192

[7] Twisting of the second transmembrane α-helix of the mitochondrial ADP/ATP carrier during the transition between two carrier conformational states [J].

Kihira, Y ;

Iwahashi, A ;

Majima, E ;

Terada, H ;

Shinohara, Y .

BIOCHEMISTRY, 2004, 43 (48) :15204-15209

[8] The ADP and ATP transport in mitochondria and its carrier [J].

Klingenberg, Martin .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2008, 1778 (10) :1978-2021

[9]

MAJIMA E, 1993, J BIOL CHEM, V268, P22181

[10]

MAJIMA E, 2000, PUMP TRANSPORTER, P179

← 1 2 →