Mammary gland amino acid flux for lactating dairy cows in response to hyperinsulinemia and dietary protein

被引:0
作者
Bompadre, T. F., V [1 ]
Campos, L. M. [1 ]
Rius, A. G. [1 ]
Kirovski, D. [2 ]
Appuhamy, J. A. D. R. N. [1 ]
Hanigan, M. D. [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Dairy Sci Dept, Blacksburg, VA 24061 USA
[2] Univ Belgrade, Dept Physiol & Biochem, Fac Vet Med, Belgrade, Serbia
来源
ENERGY AND PROTEIN METABOLISM AND NUTRITION | 2019年 / 138卷
关键词
essential; Holstein; insulin; methionine; milk protein;
D O I
10.3920/978-90-8686-891-9_121
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
This trial aimed to evaluate mammary gland amino acid (AA) flux in response to hyperinsulinemia with two levels of dietary metabolizable protein (MP). Six Holstein cows were randomly assigned in a 2x2 factorial arrangement crossover design (two MP intake levels and two blood insulin concentrations). Insulin infusions were performed to achieve hyperinsulinemia while maintaining euglycemia. Arterial and venous blood was sampled every h over 8 h and the flux of each AA was calculated and expressed as mmol/l of milk. The MP intake increased the flux of Ile (P<0.01) and tended to increase the fluxes of Lys, Phe and Val (P=0.10) from blood to mammary gland. Hyperinsulinemia increased the flux of Ala (P=0.01) and tended to increase the flux of Met (P=0.14). Lys tended to show an interaction among treatments.
引用
收藏
页码:397 / 398
页数:2
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