Characterization of badnaviruses infecting Dioscorea spp. in the Pacific reveals two putative novel species and the first report of dioscorea bacilliform RT virus 2

被引：23

作者：

Sukal, Amit ^{[1
]}

Kidanemariam, Dawit ^{[1
]}

Dale, James ^{[1
]}

James, Anthony ^{[1
]}

Harding, Robert ^{[1
]}

机构：

[1] Queensland Univ Technol, Ctr Trop Crops & Biocommod, 2 George St, Brisbane, Qld 4001, Australia

来源：

VIRUS RESEARCH | 2017年 / 238卷

关键词：

Yam; Episomal badnavirus; Rolling circle amplification; Dioscorea; Dioscorea bacilliform ES virus; Dioscorea bacilliform AL virus 2; COMPLETE GENOME SEQUENCE; BANANA-STREAK-VIRUS; MOTTLE VIRUS; PROTEIN; AMPLIFICATION; MOVEMENT; PROMOTER;

D O I：

10.1016/j.virusres.2017.05.027

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The complete genome sequences of three new badnaviruses associated with yam (Dioscorea spp.) originating from Fiji, Papua New Guinea and Samoa were determined following rolling circle amplification of the virus genomes. The full-length genomes consisted of a single molecule of circular double-stranded DNA of 8106 bp for isolate FJ14, 7871 bp for isolate PNG10 and 7426 bp for isolate SAMO1. FJ14 and PNG10 contained three open reading frames while SAMO1 had an additional open reading frame which partially overlapped the 3' end of ORF 3. Amino acid sequence analysis of ORF 3 from the three isolates confirmed the presence of conserved motifs typical of other badnaviruses. Phylogenetic analysis revealed the sequences to be closely related to other Dioscorea infecting badnaviruses. FJ14 and PNG10 appear to be new species, which we have tentatively named dioscorea bacilliform ES virus (DBESV) and dioscorea bacilliform AL virus 2 (DBALV2), respectively, while SAMO1 represents a Pacific isolate of the recently published dioscorea bacilliform RT virus 2 and is described as dioscorea bacilliform RT virus 2-[4RT] (DBRTV2-[4RT]).

引用

页码：29 / 34

页数：6

共 30 条

[1] THE CAULIFLOWER MOSAIC VIRUS-35S PROMOTER - COMBINATORIAL REGULATION OF TRANSCRIPTION IN PLANTS [J].