Phenotypic and genotypic intra-diversity among Anatolian durum wheat "Kunduru" landraces

被引:17
作者
Alsaleh, Ahmad [1 ,4 ]
Baloch, Faheem Shehzad [2 ]
Nachit, Miloudi [1 ]
Ozkan, Hakan [3 ,4 ]
机构
[1] Int Ctr Agr Res Dry Areas ICARDA, Aleppo 5466, Syria
[2] Abant Izzet Baysal Univ, Fac Agr & Nat Sci, Dept Field Crops, TR-14280 Bolu, Turkey
[3] Cukurova Univ, Dept Field Crops, Fac Agr, TR-01330 Adana, Turkey
[4] Cukurova Univ, Inst Basic & Appl Sci, Dept Biotechnol, TR-01330 Adana, Turkey
关键词
Kunduru; Seed storage protein; Simple sequence repeat; Durum wheat; Landraces; Turkey; GENETIC DIVERSITY; SSR; CULTIVARS; MARKERS; L; RESOURCES; QUALITY; AFLP;
D O I
10.1016/j.bse.2016.01.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Kunduru is an important Anatolian landrace having peculiar traits that are appreciated by farmers and breeders. 33 accessions known as Kunduru collected by ICARDA from six geographical provinces of Turkey, were used to study the phenotypic and genotypic intra-diversity. Kunduru landraces exhibited high intra-diversity for most of the studied morphological traits. GPC (12.10-14.90%), vitreousness (75-100%), TKW (31.80-56.70 g), YP (4.70-8.00 ppm), b*-value (1430-19.50), ash content (1.60-2.0%) and gluten strength (14-60 ml) showed marked variations. Gliadin and glutenin banding patterns showed high polymorphism. 65 alleles were detected with 14 SSR markers, giving a mean of 6.77 alleles per locus. The average PIC value was 0.44 and ranged from 0.11 to 0.70. The average genetic distance between pairs of landraces was 0.47 and ranged between 0.11 and 0.72. This study showed that Kunduru landraces maintains high allelic variation. PCoA indicated that eco-geographical variables have a significant effect on SSR diversity as well as morphological traits. Many of the landraces studied are in danger of disappearing from the local farmers' fields; this study demonstrates the importance of maintaining and conserving this precious genetic resources. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:9 / 16
页数:8
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