Effect of combined treatment with FK506, FTY720, and ex vivo graft irradiation in rat small bowel transplantation: Expression of mucosal addressin cell adhesion molecule-1

被引：1

作者：

Sugito, Kiminobu ^{[1
]}

Uekusa, Shota ^{[1
]}

Kawashima, Hiroyuki ^{[1
]}

Masuko, Takayuki ^{[1
]}

Furuya, Takeshi ^{[1
]}

Konuma, Noriyoshi ^{[1
]}

Ohashi, Kensuke ^{[1
]}

Inoue, Mikiya ^{[1
]}

Ikeda, Taro ^{[1
]}

Koshinaga, Tsugumichi ^{[1
]}

机构：

[1] Nihon Univ, Sch Med, Dept Pediat Surg, Itabashi Ku, Tokyo 1738610, Japan

来源：

PEDIATRIC TRANSPLANTATION | 2010年 / 14卷 / 05期

关键词：

MAdCAM-1; FK506; FTY720; ex vivo graft irradiation; small bowel transplantation; ALLOGRAFT; IMMUNOSUPPRESSANT; SURVIVAL;

D O I：

10.1111/j.1399-3046.2010.01295.x

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

We studied the effect of the combined treatment with FK506, FTY720, and ex vivo graft irradiation. Five groups of SBT animals were studied on days 3, 5, and 7 after operation (untreated, FK506, FTY720, FK506 + FTY720, FK506 + FTY720 + irradiation). Indirect immunoperoxidase staining was performed against CD4 and MAdCAM-1. The numbers of CD4 positive cells in allografts were also analyzed by flow cytometry. The graft survival was prolonged in all of the FK506- and FTY720-treated groups. SBT allografts treated by FK506 and FTY720 demonstrated less infiltration of CD4 positive cells, but the irradiation group did not show any effects on its expression. In FK506- and FTY720-treated groups, MAdCAM-1 expression on the HEVs in PPs was up-regulated, and its expression on the ECVs in the LP was down-regulated compared with other allograft groups. Irradiation did not show any effects on MAdCAM-1 expression on both HEVs in PPs and ECVs in LP. FK506 and FTY720 prevented the infiltration of CD4 positive cells, the down-regulation of MAdCAM-1 expression on HEVs in PPs, and the up-regulation of MAdCAM-1 expression on ECVs in LP during the early phase of SBT.

引用

页码：614 / 617

页数：4

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