A heterogeneous PNA-based SERS method for DNA detection

被引：129

作者：

Fabris, Laura ^{[1
]}

Dante, Mark ^{[1
]}

Braun, Gary ^{[1
]}

Lee, Seung Joon ^{[1
]}

Reich, Norbert O. ^{[1
]}

Moskovits, Martin ^{[1
]}

Nguyen, Thuc-Quyen ^{[1
]}

Bazan, Guillermo C. ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem & biochem, Dept Mat, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 19期

关键词：

D O I：

10.1021/ja0705184

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.

引用

页码：6086 / +

页数：3

共 22 条

[1] AROCA A, 2006, SURFACE ENHANCED VIB
[2] Progress in plasmonic engineering of surface-enhanced Raman-scattering substrates toward ultra-trace analysis
Baker, GA
Moore, DS
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 382 (08) : 1751 - 1770
[3] BRAUN G, UNPUB J AM CHEM SOC
[4] A PEPTIDE INTERACTION IN THE MAJOR GROOVE OF RNA RESEMBLES PROTEIN INTERACTIONS IN THE MINOR-GROOVE OF DNA
CHEN, L
FRANKEL, AD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (11) : 5077 - 5081
[5] Mercaptoammonium-monolayer-protected, water-soluble gold, silver, and palladium clusters
Cliffel, DE
Zamborini, FP
Gross, SM
Murray, RW
[J]. LANGMUIR, 2000, 16 (25) : 9699 - 9702
[6] Fuzzy nanoassemblies: Toward layered polymeric multicomposites
Decher, G
[J]. SCIENCE, 1997, 277 (5330) : 1232 - 1237
[7] Gittins DI, 2001, ANGEW CHEM INT EDIT, V40, P3001, DOI 10.1002/1521-3773(20010817)40:16<3001::AID-ANIE3001>3.0.CO
[8] 2-5
[9] SERS platforms for high density DNA arrays
Green, M
Liu, FM
Cohen, L
Köllensperger, P
Cass, T
[J]. FARADAY DISCUSSIONS, 2006, 132 : 269 - 280
[10] Single molecule detection using surface-enhanced Raman scattering (SERS)
Kneipp, K
Wang, Y
Kneipp, H
Perelman, LT
Itzkan, I
Dasari, R
Feld, MS
[J]. PHYSICAL REVIEW LETTERS, 1997, 78 (09) : 1667 - 1670

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