Positively charged calcium phosphate/polymer nanoparticles for photodynamic therapy

被引:36
作者
Klesing, J. [1 ]
Wiehe, A. [2 ]
Gitter, B. [2 ]
Graefe, S. [2 ]
Epple, M. [1 ]
机构
[1] Univ Duisburg Essen, CeNIDE, D-45117 Essen, Germany
[2] biolitec AG, D-07745 Jena, Germany
关键词
DELIVERY; NANO;
D O I
10.1007/s10856-009-3934-7
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The charge of nanoparticles influences their ability to pass through the cellular membrane, and a positive charge should be beneficial. The negative charge of calcium phosphate nanoparticles with an inner shell of carboxymethyl cellulose (CMC) was reversed by adding an outer shell of poly(ethyleneimine) (PEI) into which the photoactive dye 5,10,15,20-tetrakis(3-hydroxyphenyl)-porphyrin (mTHPP) was loaded. The aqueous dispersion of the nanoparticles was used for photodynamic therapy with HT29 cells (human colon adenocarcinoma cells), HIG-82 cells (rabbit synoviocytes), and J774A.1 cells (murine macrophages). A high photodynamic activity (killing) together with a very low dark toxicity was observed for HIG-82 and for J774.1 cells at 2 mu M dye concentration. The killing efficiency was equivalent to the pure photoactive dye that, however, needs to be administered in alcoholic solution.
引用
收藏
页码:887 / 892
页数:6
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