Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues

被引：32
作者：
Krizkova, S.
Zitka, O.
Adam, V.
Beklova, M.
Horna, A.
Svobodova, Z.
Sures, B.
Trnkova, L.
Zeman, L.
Kizek, R. [1 ]
机构：
[1] Med Univ Agr & Forestry, Dept Chem & Biochem, Fac Agron, Zemedelska 1, CS-61300 Brno, Czech Republic
[2] Masaryk Univ, Fac Sci, Dept Biochem, Brno, Czech Republic
[3] Med Univ Agr & Forestry, Fac Agron, Dept Anim Nutr & Forage Prod, Brno, Czech Republic
[4] Univ Vet & Pharmaceut Sci, Fac Vet Hyg & Ecol, Dept Vet Ecol & Environm Protect, Brno, Czech Republic
[5] Tomas Bata Univ, Fac Technol, Dept Food Engn & Chem, Zlin, Czech Republic
[6] Univ Vet & Pharmaceut Sci, Fac Med Vet, Inst Parasitol, Brno, Czech Republic
[7] Univ S Bohemia Ceske Budejovice, Res Inst Fish Culture & Hydrobiol, Vodnany, Czech Republic
[8] Univ Duisburg Essen, Angew Zool Hydrobiol, Essen, Germany
[9] Masaryk Univ, Fac Sci, Dept Chem, CS-61137 Brno, Czech Republic
来源：
CZECH JOURNAL OF ANIMAL SCIENCE | 2007年 / 52卷 / 05期
关键词：
electrochemical detection; catalytic signal; peak H; heavy metals; fish; environmental pollution;
D O I：
10.17221/2232-CJAS
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
In the present paper, we report on the use of adsorptive transfer stripping technique in connection with chronopotentiometric stripping analysis for metallothionein determination and of differential pulse anodic stripping voltammetry for lead detection in tissues of wild perch (Perca fluviatilis, n = 6) from the Svratka River in Brno, Czech Republic. Primarily, we determined the content of MT in tissues (muscles, gonads, liver and spleen) of perch. We measured the highest content of MT in spleen and liver (100-350 ng MT per gram of fresh weight). We assume that the content of MT determined in perch tissues is probably related with the age of the fish and, therefore, with their exposition to heavy metals naturally occurring in the Svratka River. We detected a lead concentration in the tissues of one perch. It clearly follows from the results that the content of MT well correlates with the concentration of lead.
引用
收藏
页码：143 / 148
页数：6
相关论文
共 38 条
[1]   Natural occurrence of metallothionein-like proteins in the liver of fish Oreochromis niloticus and effects of cadmium, lead, copper, zinc, and iron exposures on their profiles [J].
Atli, G ;
Canli, M .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2003, 70 (03) :619-627
[2]   Recent developments in quantification methods for metallothionein [J].
Dabrio, M ;
Rodríguez, AR ;
Bordin, G ;
Bebianno, MJ ;
De Ley, M ;
Sestaková, I ;
Vasák, M ;
Nordberg, M .
JOURNAL OF INORGANIC BIOCHEMISTRY, 2002, 88 (02) :123-134
[3]   Can metallothionein be a useful molecular marker for selecting hepatocellular carcinoma patients for platinum-based chemotherapy? [J].
Doki, Y ;
Monden, M .
JOURNAL OF GASTROENTEROLOGY, 2004, 39 (12) :1228-1229
[4]   Evaluation of different purification procedures for the electrochemical quantification of mussel metallothioneins [J].
Erk, M ;
Ivankovic, D ;
Raspor, B ;
Pavicic, J .
TALANTA, 2002, 57 (06) :1211-1218
[5]   Uptake and depuration of cadmium, nickel, and lead in laboratory-exposed Tubifex tubifex and corresponding changes in the concentration of a metallothionein-like protein [J].
Gillis, PL ;
Dixon, DG ;
Borgmann, U ;
Reynoldson, TB .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2004, 23 (01) :76-85
[6]   A sensor for investigating the interaction between biologically important heavy metals and glutathione [J].
Huska, D. ;
Zitka, O. ;
Adam, V. ;
Beklova, M. ;
Krizkova, S. ;
Zeman, L. ;
Horna, A. ;
Havel, L. ;
Zehnalek, J. ;
Kizek, R. .
CZECH JOURNAL OF ANIMAL SCIENCE, 2007, 52 (02) :37-43
[7]   Evaluation of the Mytilus galloprovincialis Lam. digestive gland metallothionein as a biomarker in a long-term field study:: Seasonal and spatial variability [J].
Ivankovic, D ;
Pavicic, J ;
Erk, M ;
Filipovic-Marijic, V ;
Raspor, B .
MARINE POLLUTION BULLETIN, 2005, 50 (11) :1303-1313
[8]   Comparison of two SH-based methods for estimation of metallothionein level in the digestive gland of naturally occurring mussels, Mytilus galloprovincialis [J].
Ivankovic, D ;
Pavicic, J ;
Raspor, B ;
Falnoga, I ;
Tusek-Znidaric, T .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 2003, 83 (03) :219-231
[9]   Metal components analysis of metallothionein-III in the brain sections of metallothionein-I and metallothionein-II null mice exposed to mercury vapor with HPLC/ICP-MS [J].
Kameo, S ;
Nakai, K ;
Kurokawa, N ;
Kanehisa, T ;
Naganuma, A ;
Satoh, H .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 381 (08) :1514-1519
[10]   Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine [J].
Kizek, R ;
Vacek, J ;
Trnková, L ;
Jelen, F .
BIOELECTROCHEMISTRY, 2004, 63 (1-2) :19-24
1234