Lipopolysaccharide induces apoptosis in a bovine endothelial cell line via a soluble CD14 dependent pathway

被引：68

作者：

Frey, EA

Finlay, BB

机构：

[1] Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada

[2] Univ British Columbia, Dept Immunol & Microbiol, Vancouver, BC V6T 1Z3, Canada

[3] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V6T 1Z3, Canada

来源：

MICROBIAL PATHOGENESIS | 1998年 / 24卷 / 02期

关键词：

lipopolysaccharide (LPS); apoptosis; soluble CD14 (sCD14); endothelium;

D O I：

10.1006/mpat.1997.0178

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the mode of bovine endothelial cell death induced by lipopolysaccharide (LPS). Inter-nucleosomal DNA fragmentation was observed indicating apoptotic cell death. Apoptosis was confirmed by examining cell morphology using confocal laser scanning and transmission electron microscopy, which revealed characteristic nuclear condensation in LPS-treated endothelial cells. Apoptosis was blocked by a monoclonal antibody against CD14, suggesting that LPS triggers apoptosis via a soluble CD14 (sCD14) dependent mechanism. (C) 1998 Academic Press Limited.

引用

页码：101 / 109

页数：9

共 37 条

[1] ENDOTOXIN-MEDIATED ENDOTHELIAL-CELL INJURY AND ACTIVATION - ROLE OF SOLUBLE CD14 [J].