Characterization of the ars Gene Cluster from Extremely Arsenic-Resistant Microbacterium sp Strain A33

被引：57

作者：

Achour-Rokbani, Asma ^{[2
]}

Cordi, Audrey ^{[2
]}

Poupin, Pascal ^{[2
]}

Bauda, Pascale ^{[2
]}

Billard, Patrick ^{[1
,2
]}

机构：

[1] Nancy Univ, CNRS, LIMOS, UHP,UMR 7137, F-54506 Vandoeuvre Les Nancy, France

[2] Univ Paul Verlaine Metz, CNRS, LIEBE, UMR 7146, F-57070 Metz, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 03期

关键词：

ESCHERICHIA-COLI; CORYNEBACTERIUM-GLUTAMICUM; REDUCTION; TRANSFORMATION; BACTERIA; PROTEIN; SYSTEM; EXPRESSION; MYCOTHIOL; DIVERSITY;

D O I：

10.1128/AEM.01738-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The arsenic resistance gene cluster of Microbacterium sp. A33 contains a novel pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole ars gene cluster is required to complement an Escherichia coli ars mutant. ArsRC2 negatively regulates the expression of the pentacistronic operon. ArsC1 and ArsC3 are related to thioredoxin-dependent arsenate reductases; however, ArsC3 lacks the two distal catalytic cysteine residues of this class of enzymes.

引用

页码：948 / 955

页数：8