Characterization of the ars Gene Cluster from Extremely Arsenic-Resistant Microbacterium sp Strain A33

被引:56
作者
Achour-Rokbani, Asma [2 ]
Cordi, Audrey [2 ]
Poupin, Pascal [2 ]
Bauda, Pascale [2 ]
Billard, Patrick [1 ,2 ]
机构
[1] Nancy Univ, CNRS, LIMOS, UHP,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
[2] Univ Paul Verlaine Metz, CNRS, LIEBE, UMR 7146, F-57070 Metz, France
关键词
ESCHERICHIA-COLI; CORYNEBACTERIUM-GLUTAMICUM; REDUCTION; TRANSFORMATION; BACTERIA; PROTEIN; SYSTEM; EXPRESSION; MYCOTHIOL; DIVERSITY;
D O I
10.1128/AEM.01738-09
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The arsenic resistance gene cluster of Microbacterium sp. A33 contains a novel pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole ars gene cluster is required to complement an Escherichia coli ars mutant. ArsRC2 negatively regulates the expression of the pentacistronic operon. ArsC1 and ArsC3 are related to thioredoxin-dependent arsenate reductases; however, ArsC3 lacks the two distal catalytic cysteine residues of this class of enzymes.
引用
收藏
页码:948 / 955
页数:8
相关论文
共 36 条
  • [21] Isolation and Characterization of a car Gene Cluster from the Naphthalene, Phenanthrene, and Carbazole-Degrading Marine Isolate Lysobacter sp Strain OC7
    Maeda, Rintaro
    Nagashima, Hiroshi
    Bin Zulkharnain, Azham
    Iwata, Kenichi
    Omori, Toshio
    CURRENT MICROBIOLOGY, 2009, 59 (02) : 154 - 159
  • [22] Isolation and characterization of Staphylococcus sp. strain NBRIEAG-8 from arsenic contaminated site of West Bengal
    Srivastava, Shubhi
    Verma, Praveen C.
    Singh, Ankit
    Mishra, Manisha
    Singh, Namrata
    Sharma, Neeta
    Singh, Nandita
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 95 (05) : 1275 - 1291
  • [23] Isolation and characterization of Staphylococcus sp. strain NBRIEAG-8 from arsenic contaminated site of West Bengal
    Shubhi Srivastava
    Praveen C. Verma
    Ankit Singh
    Manisha Mishra
    Namrata Singh
    Neeta Sharma
    Nandita Singh
    Applied Microbiology and Biotechnology, 2012, 95 : 1275 - 1291
  • [24] Characterization and Transcriptome Studies of Autoinducer Synthase Gene from Multidrug Resistant Acinetobacter baumannii Strain 863
    Ng, Chung-Kiat
    How, Kah-Yan
    Tee, Kok-Keng
    Chan, Kok-Gan
    GENES, 2019, 10 (04)
  • [25] Identification and characterization of an ectoine biosynthesis gene cluster from Aestuariispira ectoiniformans sp. nov., isolated from seawater
    Kang, Ji Young
    Lee, Binna
    Kim, Jeong Ah
    Kim, Min-Soo
    Kim, Chul Ho
    MICROBIOLOGICAL RESEARCH, 2022, 254
  • [26] Characterization of a Cd2+-Resistant Strain of Ochrobactrum sp Isolated from Slag Disposal Site of an Iron and Steel Factory
    Pandey, Sanjeev
    Saha, Pradipta
    Barai, Prabir Kumar
    Maiti, Tushar K.
    CURRENT MICROBIOLOGY, 2010, 61 (02) : 106 - 111
  • [27] Cloning of the novel gene encoding β-agarase C from a marine bacterium, Vibrio sp strain PO-303, and characterization of the gene product
    Dong, Jinhua
    Hashikawa, Shinnosuke
    Konishi, Takafumi
    Tamaru, Yutaka
    Araki, Toshiyoshi
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (09) : 6399 - 6401
  • [28] Characterization of the arsenite oxidizer Aliihoeflea sp strain 2WW and its potential application in the removal of arsenic from groundwater in combination with Pf-ferritin
    Corsini, Anna
    Colombo, Milena
    Muyzer, Gerard
    Cavalca, Lucia
    ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2015, 108 (03): : 673 - 684
  • [29] Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabena sp. strain PCC 7120
    Stricker, O
    Masepohl, B
    Klipp, W
    Bohme, H
    JOURNAL OF BACTERIOLOGY, 1997, 179 (09) : 2930 - 2937
  • [30] Cloning and characterization of a novel gene encoding L-ribose isomerase from Acinetobacter sp strain DL-28 in Escherichia coli
    Mizanur, RM
    Takata, G
    Izumori, K
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2001, 1521 (1-3): : 141 - 145