Influence of hormonal and nonhormonal estrus synchronization methods on follicular and oocyte quality in primiparous lactating does at early postpartum period

被引:14
作者
Arias-Alvarez, M. [1 ]
Garcia-Garcia, R. M. [1 ]
Torres-Rovira, L. [2 ]
Gonzalez-Bulnes, A. [2 ]
Rebollar, P. G. [3 ]
Lorenzo, P. L. [1 ]
机构
[1] Univ Complutense Madrid, Fac Vet, Dept Fisiol Fisiol Anim, Madrid, Spain
[2] INIA, Dept Reprod Anim & Conservac Recursos Zoogenet, Madrid, Spain
[3] Univ Politecn Madrid, Dept Anim Prod, ETSI Agronomos, Madrid, Spain
关键词
eCG; Follicular atresia; Oocyte maturation; Transient doe-litter separation; Steroidogenesis; GRANULOSA-CELL APOPTOSIS; DOE-LITTER SEPARATION; IN-VITRO MATURATION; GROWTH-FACTOR-I; STIMULATING-HORMONE; DEVELOPMENTAL COMPETENCE; OVARIAN RESPONSE; BOVINE OOCYTES; LUTEINIZING-HORMONE; SEXUAL RECEPTIVITY;
D O I
10.1016/j.theriogenology.2009.07.017
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
High-yield lactating does need effective estrus synchronization methods to improve their reproductive outcome by enhancing ovarian function. The aim of the current work was to analyze ovarian follicular and oocyte characteristics of hormonal and nonhormonal estrus synchronization regimes in primiparous lactating rabbit does (Oryctolagus cuniculus) in the early postpartum period (Day 11). Females were randomly treated with either (1) a hormonal standard treatment with 25 IU equine chorionic gonadotropin (eCG) 48 h before artificial insemination (eCG group) or (2) an alternative nonhormonal treatment consisting of doe-litter separation 24 h before artificial insemination (Bio group). No significant differences were found in serum estradiol and progesterone concentrations between experimental groups. During the histologic study, the Bio group presented a higher number of primordial (P < 0.05) and primary follicles (P = 0.07) compared with that of the eCG group, whereas secondary and antral follicular populations were similar. Rates of late atretic follicles assessed by the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling technique were not different between treatments, but the eCG group showed a significantly higher number of mid-atretic follicles compared with that of the Bio group. Nuclear in vitro oocyte maturation (IVM), measured as metaphase II rate, and in vitro steroidogenic response of cumulus-oocyte complexes, measured by ELISA, did not show significant differences between treatments. However, confocal study showed that cytoplasmic maturation of oocytes, in terms of cortical granule migration rate, was significantly higher in the Bio group compared with that after the eCG treatment. In conclusion, transient doe-litter separation seems to improve ovarian response in terms of follicular health and oocyte competence compared with that after the eCG treatment. Therefore, a 24-h-long transient weaning could be an alternative nonhormonal method for synchronizing estrus in primiparous lactating rabbit does inseminated in the early postpartum period. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:26 / 35
页数:10
相关论文
共 73 条
[1]  
Alvarino J. M. R., 1998, World Rabbit Science, V6, P191
[3]  
[Anonymous], [No title captured]
[4]  
[Anonymous], ITEA
[5]   Influence of metabolic status on oocyte quality and follicular characteristics at different postpartum periods in primiparous rabbit does [J].
Arias-Alvarez, M. ;
Garcia-Garcia, R. M. ;
Rebollar, P. G. ;
Revuelta, L. ;
Millan, P. ;
Lorenzo, P. L. .
THERIOGENOLOGY, 2009, 72 (05) :612-623
[6]  
ARIASALVAREZ M, 2009, LIVEST SCI, V121, P227
[7]   OOCYTE STRUCTURE AND FOLLICULAR STEROID CONCENTRATIONS IN SUPEROVULATED VERSUS UNSTIMULATED HEIFERS [J].
ASSEY, RJ ;
HYTTEL, P ;
ROCHE, JF ;
BOLAND, M .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1994, 39 (01) :8-16
[8]   PROGESTINS INHIBIT MURINE OOCYTE MEIOTIC MATURATION INVITRO [J].
BARRETT, CB ;
POWERS, RD .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1993, 265 (03) :231-239
[9]   OOCYTE AND FOLLICULAR MORPHOLOGY AS DETERMINING CHARACTERISTICS FOR DEVELOPMENTAL COMPETENCE IN BOVINE OOCYTES [J].
BLONDIN, P ;
SIRARD, MA .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1995, 41 (01) :54-62
[10]  
Boiti C., 1995, World Rabbit Science, V3, P51