DISSIPATION OF DOUBLE-STRANDED RNA IN AQUATIC MICROCOSMS

被引：31
作者：
Albright, Vurtice C., III [1 ]
Wong, Colin R. [1 ]
Hellmich, Richard L. [2 ]
Coats, Joel R. [1 ]
机构：
[1] Iowa State Univ, Dept Entomol, Pesticide Toxicol Lab, Ames, IA USA
[2] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA USA
来源：
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY | 2017年 / 36卷 / 05期
关键词：
dsRNA; Double-stranded RNA; Environmental fate; Aquatic microcosms; BACILLUS-THURINGIENSIS; TARGETED ACTIVITY; DNA; INTERFERENCE; PERSISTENCE; FATE; GENE; SOIL; DEGRADATION; DSRNA;
D O I：
10.1002/etc.3648
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Silencing genes of a pest with double-stranded RNA (dsRNA) is a promising new pest management technology. As part of the environmental risk assessment for dsRNA-based products, the environmental fate and the potential for adverse effects to on-target organisms should be characterized. In the present study, a nonbioactive dsRNA was spiked into the water column of a water and sediment microcosm to mimic drift from a spray application run off of unbound dsRNA or transport of plant tissues. Dissipation of dsRNA in the water column and partitioning into sediment was determined. The dsRNA rapidly dissipated in the water column and was below the limit of detection after 96 h. The levels detected in the sediment were not significant and may indicate rapid degradation in the water column prior to partitioning to sediment. (C) 2016 SETAC
引用
收藏
页码：1249 / 1253
页数：5
相关论文
共 38 条
  • [1] Ecological risk assessment for DvSnf7 RNA: A plant-incorporated protectant with targeted activity against western corn rootworm
    Bachman, Pamela M.
    Huizinga, Kristin M.
    Jensen, Peter D.
    Mueller, Geoffrey
    Tan, Jianguo
    Uffman, Joshua P.
    Levine, Steven L.
    [J]. REGULATORY TOXICOLOGY AND PHARMACOLOGY, 2016, 81 : 77 - 88
  • [2] Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)
    Bachman, Pamela M.
    Bolognesi, Renata
    Moar, William J.
    Mueller, Geoffrey M.
    Paradise, Mark S.
    Ramaseshadri, Parthasarathy
    Tan, Jianguo
    Uffman, Joshua P.
    Warren, JoAnne
    Wiggins, B. Elizabeth
    Levine, Steven L.
    [J]. TRANSGENIC RESEARCH, 2013, 22 (06) : 1207 - 1222
  • [3] BAUM JA, 2014, ADV INSECT PHYSIOL, V47, P249, DOI DOI 10.1016/B978-0-12-800197-4.00005-1
  • [4] Control of coleopteran insect pests through RNA interference
    Baum, James A.
    Bogaert, Thierry
    Clinton, William
    Heck, Gregory R.
    Feldmann, Pascale
    Ilagan, Oliver
    Johnson, Scott
    Plaetinck, Geert
    Munyikwa, Tichafa
    Pleau, Michael
    Vaughn, Ty
    Roberts, James
    [J]. NATURE BIOTECHNOLOGY, 2007, 25 (11) : 1322 - 1326
  • [5] Characterizing the Mechanism of Action of Double-Stranded RNA Activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)
    Bolognesi, Renata
    Ramaseshadri, Parthasarathy
    Anderson, Jerry
    Bachman, Pamela
    Clinton, William
    Flannagan, Ronald
    Ilagan, Oliver
    Lawrence, Christina
    Levine, Steven
    Moar, William
    Mueller, Geoffrey
    Tan, Jianguo
    Uffman, Joshua
    Wiggins, Elizabeth
    Heck, Gregory
    Segers, Gerrit
    [J]. PLOS ONE, 2012, 7 (10):
  • [6] FIELD DISSIPATION OF FOUR PERSONAL CARE PRODUCTS IN BIOSOLIDS-AMENDED SOILS IN NORTH CHINA
    Chen, Feng
    Ying, Guang-Guo
    Ma, Yi-Bing
    Chen, Zhi-Feng
    Lai, Hua-Jie
    [J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2014, 33 (11) : 2413 - 2421
  • [7] FATE AND EFFECTS OF CLOTHIANIDIN IN FIELDS USING CONSERVATION PRACTICES
    de Perre, Chloe
    Murphy, Tracye M.
    Lydy, Michael J.
    [J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2015, 34 (02) : 258 - 265
  • [8] Environmental Fate of Double-Stranded RNA in Agricultural Soils
    Dubelman, Samuel
    Fischer, Joshua
    Zapata, Fatima
    Huizinga, Kristin
    Jiang, Changjian
    Uffman, Joshua
    Levine, Steven
    Carson, David
    [J]. PLOS ONE, 2014, 9 (03):
  • [9] Occurrence, fate, and persistence of gemfibrozil in water and soil
    Fang, Yu
    Karnjanapiboonwong, Adcharee
    Chase, Darcy A.
    Wang, Jiafan
    Morse, Audra N.
    Anderson, Todd A.
    [J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2012, 31 (03) : 550 - 555
  • [10] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    Fire, A
    Xu, SQ
    Montgomery, MK
    Kostas, SA
    Driver, SE
    Mello, CC
    [J]. NATURE, 1998, 391 (6669) : 806 - 811
1234