Evolutionary relationships between the former species Saccharomyces uvarum and the hybrids Saccharomyces bayanus and Saccharomyces pastorianus;: reinstatement of Saccharomyces uvarum (Beijerinck) as a distinct species

被引：72
作者：
Nguyen, HV [1 ]
Gaillardin, C [1 ]
机构：
[1] Ctr Versailles Grignon, INA PG, Lab Microbiol & Genet Mol,CNRS,UMR2585, Collect Levures Interet Biotechnol,INRA,UMR1238, F-78850 Thiverval Grignon, France
来源：
FEMS YEAST RESEARCH | 2005年 / 5卷 / 4-5期
关键词：
Saccharomyces bayanus; Saccharomyces uvarum; Saccharomyces pastorianus; taxonomy; GDH1; MET2; BAP2;
D O I：
10.1016/j.femsyr.2004.12.004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Analysis of the nucleotide sequence of the GDH1 homologues from Saccharomyces bayanus strain CBS 380(T) and S. pastorianus strains showed that they share an almost identical sequence, SuGDH1*, which is a diverged form of the SuGDH1 from the type strain of the former species S. uvarum, considered as synonym of S. bayanus. SuGDH1* is close to but differs from SuGDH1 by the accumulation of a high number of neutral substitutions designated as Multiple Neutral Mutations Accumulation (MNMA). Further analysis carried out with three other markers, BAP2, HO and MET2 showed that they have also diverged from their S. uvarum counterparts by MNMA. S. bayanus CBS 380T is placed between S. uvarum and S. pastorianus sharing MET2, CDC91 sequences with the former and BAP2, GDH1, HO sequences with the latter. S. bayanus CBS 380T has been proposed to be a S. uvarum/S. cerevisiae hybrid and this proposal is confirmed by the presence in its genome a S. cerevisiae SUC4 gene. Strain S. bayanus CBS 380T, with a composite genome, is genetically isolated from strains of the former S. uvarum species, thus justifying the reinstatement of S. uvarum as a distinct species. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：471 / 483
页数：13
相关论文
共 39 条
[1]   Genomic Exploration of the Hemiascomycetous Yeasts:: 5.: Saccharomyces bayanus var. uvarum [J].
Bon, E ;
Neuvéglise, C ;
Casaregola, S ;
Artiguenave, F ;
Wincker, P ;
Aigle, M ;
Durrens, P .
FEBS LETTERS, 2000, 487 (01) :37-41
[2]  
Borsting C, 1997, YEAST, V13, P1409, DOI 10.1002/(SICI)1097-0061(199712)13:15<1409::AID-YEA188>3.3.CO
[3]  
2-1
[4]   EVOLUTION OF THE DISPERSED SUC GENE FAMILY OF SACCHAROMYCES BY REARRANGEMENTS OF CHROMOSOME TELOMERES [J].
CARLSON, M ;
CELENZA, JL ;
ENG, FJ .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (11) :2894-2902
[5]   Analysis of the constitution of the beer yeast genome by PCR, sequencing and subtelomeric sequence hybridization [J].
Casaregola, S ;
Nguyen, HV ;
Lapathitis, G ;
Kotyk, A ;
Gaillardin, C .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1607-1618
[6]   Finding functional features in Saccharomyces genomes by phylogenetic footprinting [J].
Cliften, P ;
Sudarsanam, P ;
Desikan, A ;
Fulton, L ;
Fulton, B ;
Majors, J ;
Waterston, R ;
Cohen, BA ;
Johnston, M .
SCIENCE, 2003, 301 (5629) :71-76
[7]   A SEQUENCE ASSEMBLY AND EDITING PROGRAM FOR EFFICIENT MANAGEMENT OF LARGE PROJECTS [J].
DEAR, S ;
STADEN, R .
NUCLEIC ACIDS RESEARCH, 1991, 19 (14) :3907-3911
[8]   Analysis of the genetic variability in the species of the Saccharomyces sensu stricto complex [J].
Fernández-Espinar, MT ;
Barrio, E ;
Querol, A .
YEAST, 2003, 20 (14) :1213-1226
[9]   Karyotyping of Saccharomyces strains with different temperature profiles [J].
Giudici, P. ;
Caggia, C. ;
Pulvirenti, A. ;
Rainieri, S. .
1998, (84)
[10]  
Groth G, 1999, INT J SYST BACTERIOL, V49, P1933
1234