The prevalence of Candida in bloodstream infections (BSIs) has increased. To date, the identification of Candida in BSIs still mainly relies on blood culture and serological tests, but they have various limitations. Therefore, a real-time PCR assay for the detection of Candida from whole blood is presented. The unique primers/probe system was designed on 5.8S rRNA gene (5.8S rDNA) of Candida genus. The analytical sensitivity was determined by numbers of positive PCRs in 12 repetitions. At the concentration of 10(1) CFU/ml blood, positive PCR rates of 100 % were obtained for C. albicans, C. parapsilosis, C. tropicalis, and C. krusei. The detection rate for C. glabrata was 75 % at 10(1) CFU/ml blood. The reaction specificity was 100 % when evaluating the assay using DNA samples from clinical isolates and human blood. The maximum CVs of intra-assay and inter-assay for the detection limit were 1.22 and 2.22 %, respectively. To assess the clinical applicability, 328 blood samples from 82 patients were prospectively tested and real-time PCR results were compared with results from blood culture. Diagnostic sensitivity of the PCR was 100 % using as gold standard blood culture, and specificity was 98.4 %. Our data suggest that the developed assay can be used in clinical laboratories as an accurate and rapid screening test for the Candida from whole blood. Although further evaluation is warranted, our assay holds promise for earlier diagnosis of candidemia.
机构:
Tel Aviv Univ, Beilinson Hosp, Rabin Med Ctr, Med E, IL-49100 Petah Tiqwa, IsraelTel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
Avni, Tomer
Leibovici, Leonard
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Tel Aviv Univ, Beilinson Hosp, Rabin Med Ctr, Med E, IL-49100 Petah Tiqwa, IsraelTel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
Leibovici, Leonard
Paul, Mical
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Tel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
Tel Aviv Univ, Rabin Med Ctr, Infect Dis Unit, Beilinson Hosp, IL-49100 Petah Tiqwa, IsraelTel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
机构:
Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Chen, L. -Y.
Liao, S. -Y.
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Taipei Vet Gen Hosp, Infect Control, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Liao, S. -Y.
Kuo, S. -C.
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Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Kuo, S. -C.
Chen, S. -J.
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Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Chen, S. -J.
Chen, Y. -Y.
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Taipei Vet Gen Hosp, Infect Control, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Chen, Y. -Y.
Wang, F. -D.
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Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Taipei Vet Gen Hosp, Infect Control, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Wang, F. -D.
Yang, S. -P.
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Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Yang, S. -P.
Fung, C. -P.
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Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
机构:
VA Pittsburgh Healthcare Syst, Dept Med, Pittsburgh, PA USA
Univ Pittsburgh, Pittsburgh, PA 15261 USAVA Pittsburgh Healthcare Syst, Dept Med, Pittsburgh, PA USA
Clancy, Cornelius J.
Nguyen, M. Hong
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Univ Pittsburgh, Pittsburgh, PA 15261 USAVA Pittsburgh Healthcare Syst, Dept Med, Pittsburgh, PA USA
机构:
Tel Aviv Univ, Beilinson Hosp, Rabin Med Ctr, Med E, IL-49100 Petah Tiqwa, IsraelTel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
Avni, Tomer
Leibovici, Leonard
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机构:
Tel Aviv Univ, Beilinson Hosp, Rabin Med Ctr, Med E, IL-49100 Petah Tiqwa, IsraelTel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
Leibovici, Leonard
Paul, Mical
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Tel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
Tel Aviv Univ, Rabin Med Ctr, Infect Dis Unit, Beilinson Hosp, IL-49100 Petah Tiqwa, IsraelTel Aviv Univ, Sackler Fac Med, IL-49100 Tel Aviv, Israel
机构:
Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Chen, L. -Y.
Liao, S. -Y.
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h-index: 0
机构:
Taipei Vet Gen Hosp, Infect Control, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Liao, S. -Y.
Kuo, S. -C.
论文数: 0引用数: 0
h-index: 0
机构:
Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Kuo, S. -C.
Chen, S. -J.
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h-index: 0
机构:
Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Chen, S. -J.
Chen, Y. -Y.
论文数: 0引用数: 0
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机构:
Taipei Vet Gen Hosp, Infect Control, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Chen, Y. -Y.
Wang, F. -D.
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h-index: 0
机构:
Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Taipei Vet Gen Hosp, Infect Control, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Wang, F. -D.
Yang, S. -P.
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机构:
Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Yang, S. -P.
Fung, C. -P.
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机构:
Taipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
Natl Yang Ming Univ, Sch Med, Taipei 112, TaiwanTaipei Vet Gen Hosp, Dept Med, Div Infect Dis, Taipei 11217, Taiwan
机构:
VA Pittsburgh Healthcare Syst, Dept Med, Pittsburgh, PA USA
Univ Pittsburgh, Pittsburgh, PA 15261 USAVA Pittsburgh Healthcare Syst, Dept Med, Pittsburgh, PA USA
Clancy, Cornelius J.
Nguyen, M. Hong
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h-index: 0
机构:
Univ Pittsburgh, Pittsburgh, PA 15261 USAVA Pittsburgh Healthcare Syst, Dept Med, Pittsburgh, PA USA