Liquid chromatographic retention behavior and enantioseparation of fluoxetine on a new β-cyclodextrin bonded-phase column

A new beta -cyclodextrin phenyl isocyanate bonded stationary phase had been used to investigate the retention time, resolution, separation factor of fluoxetine under the different LC condition in the reverse phase. The optimal operation conditions were found. A base line separation of the enantiomers had been obtained. In the paper, the effect of mobile phase composition, ionic strength in the mobile phase, flow rate, pH and temperature on retention time, resolution and selectivity of R-FLU and S-FLU had been discussed. The experiment results show that these factors can all affect retention time and resolution, but have no or slight effect on the selectivity. The separation result is better than that reported before. when methanol-buffer ratio is 40:60, flow rate is 0.5 ml/min, the concentration of triethylamine in the mobile phase is 2%, pH is 5.0, and the temperature is 23 degreesC, the retention time is 29.02 min and 25.72 min, respectively, the resolution is 1.130, the selectivity is 1.187.