Effect of several PEO-PPO amphiphiles on bax, bcl-2, and hTERT mRNAs: An insight into apoptosis and cell immortalization induced in hepatoma cells by these polymeric excipients

被引：4
作者：
Celeste Castronuovo, Cynthia [1 ,2 ]
Lujan Cuestas, Maria [1 ,2 ]
Raul Oubina, Jose [1 ,2 ]
Lidia Mathet, Veronica [1 ,2 ]
机构：
[1] Univ Buenos Aires, UBA CONICET, Fac Med, Inst Invest Microbiol & Parasitol Med, RA-1053 Buenos Aires, DF, Argentina
[2] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina
来源：
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2016年 / 63卷 / 02期
关键词：
bax; bcl-2; hTERT; multiplex RT-PCR; poloxamines; poloxamers; TELOMERASE ACTIVITY; IN-VIVO; CANCER; EXPRESSION; DRUG; COPOLYMERS; PATHWAYS;
D O I：
10.1002/bab.1352
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent data have shown that synthetic polymers and nanomaterials display phenotypic effects in cells and signal transduction mechanisms involved in inflammation, differentiation, proliferation, and apoptosis. Aim: This article aims to investigate the effect of poly(ethylene oxide)-poly(propylene oxide) (PEO-PPO) block copolymers with a wide range of biomedical and pharmaceutical applications on apoptosis and/or cell immortalization, by flow cytometry and multiplex RT-PCR for bax, bcl-2, and human telomerase reverse transcriptase (hTERT). Results: PEO-PPO amphiphiles upregulated bax and hTERT and induced apoptosis of two human hepatoma cell lines. Conclusions: PEO-PPO block copolymersconsidered safe for human usecan drastically alter gene expression profiles of genes related to apoptosis/cell proliferation.
引用
收藏
页码：273 / 280
页数：8
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