Application of Three-dimensional (3D) Tumor Cell Culture Systems and Mechanism of Drug Resistance

被引：25
作者：
Shehzad, Adeeb [1 ]
Ravinayagam, Vijaya [2 ,3 ]
AlRumaih, Hamad [4 ]
Aljafary, Meneerah [5 ]
AlMuhazay, Dana [6 ]
Almofty, Sara [6 ]
Al-Rashid, Noor A. [1 ]
Al-Suhaimi, Ebtesam [5 ,6 ]
机构：
[1] Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat, Dept Clin Pharm, Dammam, Saudi Arabia
[2] Sci Res, Dammam, Saudi Arabia
[3] Imam Abdulrahman Bin Faisal Univ, Dammam, Saudi Arabia
[4] Imam Abdulrahman Bin Faisal Univ, Coll Dent, Dammam, Saudi Arabia
[5] Imam Abdulrahman Bin Faisal Univ, Coll Sci, Dept Biol, Dammam, Saudi Arabia
[6] Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat, Stem Cell Res Dept, Dammam, Saudi Arabia
来源：
CURRENT PHARMACEUTICAL DESIGN | 2019年 / 25卷 / 34期
关键词：
Spheroid; 3D cell culture; drug resistance; drug discovery; cancer therapy; 2D monolayer; IN-VITRO; MULTICELLULAR SPHEROIDS; STROMAL CELLS; CANCER; CURCUMIN; MODEL; DISCOVERY; ENRICHMENT; GROWTH; PHARMACOKINETICS;
D O I：
10.2174/1381612825666191014163923
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The in-vitro experimental model for the development of cancer therapeutics has always been challenging. Recently, the scientific revolution has improved cell culturing techniques by applying three dimensional (3D) culture system, which provides a similar physiologically relevant in-vivo model for studying various diseases including cancer. In particular, cancer cells exhibiting in-vivo behavior in a model of 3D cell culture is a more accurate cell culture model to test the effectiveness of anticancer drugs or characterization of cancer cells in comparison with two dimensional (2D) monolayer. This study underpins various factors that cause resistance to anti-cancer drugs in forms of spheroids in 3D in-vitro cell culture and also outlines key challenges and possible solutions for the future development of these systems.
引用
收藏
页码：3599 / 3607
页数：9
相关论文
共 93 条
[31]   The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting [J].
Ito, A ;
Ino, K ;
Kobayashi, T ;
Honda, H .
BIOMATERIALS, 2005, 26 (31) :6185-6193
[32]   Three-Dimensional In Vitro Co-Culture Model of Breast Tumor using Magnetic Levitation [J].
Jaganathan, Hamsa ;
Gage, Jacob ;
Leonard, Fransisca ;
Srinivasan, Srimeenakshi ;
Souza, Glauco R. ;
Dave, Bhuvanesh ;
Godin, Biana .
SCIENTIFIC REPORTS, 2014, 4
[33]   Cell cultures in drug discovery and development: The need of reliable in vitro-in vivo extrapolation for pharmacodynamics and pharmacokinetics assessment [J].
Jaroch, Karol ;
Jaroch, Alina ;
Bojko, Barbara .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2018, 147 :297-312
[34]   Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types [J].
Kelm, JM ;
Timmins, NE ;
Brown, CJ ;
Fussenegger, M ;
Nielsen, LK .
BIOTECHNOLOGY AND BIOENGINEERING, 2003, 83 (02) :173-180
[35]   General functionalization route for cell adhesion on non-wetting surfaces [J].
Ku, Sook Hee ;
Ryu, Jungki ;
Hong, Seon Ki ;
Lee, Haeshin ;
Park, Chan Beum .
BIOMATERIALS, 2010, 31 (09) :2535-2541
[36]   Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning [J].
Langhans, Sigrid A. .
FRONTIERS IN PHARMACOLOGY, 2018, 9
[37]   Modulation of Huh7.5 Spheroid Formation and Functionality Using Modified PEG-Based Hydrogels of Different Stiffness [J].
Lee, Bae Hoon ;
Kim, Myung Hee ;
Lee, Jae Ho ;
Seliktar, Dror ;
Cho, Nam-Joon ;
Tan, Lay Poh .
PLOS ONE, 2015, 10 (02)
[38]   A three-dimensional microenvironment alters protein expression and chemosensitivity of epithelial ovarian cancer cells in vitro [J].
Lee, Janet Myungjin ;
Mhawech-Fauceglia, Paulette ;
Lee, Nathan ;
Parsanian, Lucineh Cristina ;
Lin, Yvonne Gail ;
Gayther, Simon Andrew ;
Lawrenson, Kate .
LABORATORY INVESTIGATION, 2013, 93 (05) :528-542
[39]   In vitro Toxicity Testing of Nanoparticles in 3D Cell Culture [J].
Lee, Jungwoo ;
Lilly, G. Daniel ;
Doty, R. Christopher ;
Podsiadlo, Paul ;
Kotov, Nicholas A. .
SMALL, 2009, 5 (10) :1213-1221
[40]   Protein Kinase Cα Protects Against Multidrug Resistance in Human Colon Cancer Cells [J].
Lee, Se-Kyoung ;
Shehzad, Adeeb ;
Jung, Jae-Chang ;
Sonn, Jong-Kyung ;
Lee, Jae-Tae ;
Park, Jeen-Woo ;
Lee, Young-Sup .
MOLECULES AND CELLS, 2012, 34 (01) :61-69
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