First report of Tomato leaf curl Joydebpur virus infecting French bean (Phaseolus vulgaris L.)

被引:6
|
作者
Ansar, Mohammad [1 ]
Agnihotri, Aniruddha Kumar [1 ]
Akram, Mohammad [2 ]
Bhagat, Arun Prasad [1 ]
机构
[1] Bihar Agr Univ, Dept Plant Pathol, Bhagalpur 813210, Bihar, India
[2] Indian Inst Pulses Res, Div Crop Protect, Kanpur 208024, Uttar Pradesh, India
关键词
Bean; Begomovirus; Betasatellite; Leaf curl; Whitefly; MOLECULAR CHARACTERIZATION; DNA-BETA; BEGOMOVIRUS; GEMINIVIRUS; DISEASE; PLANTS; IDENTIFICATION; BETASATELLITE; INSECTS;
D O I
10.1007/s10327-019-00867-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
During 2016-2017 in Bhagalpur, Bihar, northern India, French bean plants developed curling and yellow mosaic of leaves and stunting. In eight of 12 symptomatic plants, PCR tests for Mungbean yellow mosaic India virus were positive. In the four PCR-negative symptomatic plants, rolling circle amplification (RCA) detected a similar to 2.7-kb DNA that was identified as genomic DNA of Tomato leaf curl Joydebpur virus. Also a similar to 1.4-kb specific PCR product was identified as Tomato leaf curl Joydebpur betasatellite. Whitefly-mediated transmission to chili was as efficient as that to French bean. Our findings provide evidence of a monopartite begomovirus in French bean.
引用
收藏
页码:444 / 448
页数:5
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