Methylation of histone H3 in euchromatin of plant chromosomes depends on basic nuclear DNA content

被引：128

作者：

Houben, A ^{[1
]}

Demidov, D ^{[1
]}

Gernand, D ^{[1
]}

Meister, A ^{[1
]}

Leach, CR ^{[1
]}

Schubert, I ^{[1
]}

机构：

[1] Inst Plant Genet & Crop Plant Res, IPK, D-06466 Gatersleben, Germany

来源：

PLANT JOURNAL | 2003年 / 33卷 / 06期

关键词：

euchromatin; heterochromatin; genome size; histone H3 methylation; silencing;

D O I：

10.1046/j.1365-313X.2003.01681.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Strong methylation of lysine 4 (K4) and low methylation of lysine 9 (K9) have been proposed as modifications of histone H3, typical for transcriptionally active euchromatin and the opposite for inactive heterochromatin. We have analysed the correlation between the global distribution of histone H3, methylated at either lysine 4 or lysine 9, and of microscopically detectable euchromatic or heterochromatic regions in relation to genome size for 24 plant species. Two different distribution patterns of methylated (K9)H3 (Met(K9)H3) were found that depend on genome size. For most species with small genomes (1C <500 Mbp), including Arabidopsis thaliana, strong methylation of (K9)H3 was restricted to constitutive heterochromatin. Species with larger genomes showed a uniform distribution of Met(K9)H3. Contrary to this and regardless of the genome size, methylated (K4)H3 (Met(K4)H3) was found to be enriched within the euchromatin of all species. Transcriptionally less active B chromosomes showed the same patterns as basic A chromosomes. We thus propose that large genomes with high amounts of dispersed repetitive sequences (mainly retroelements) have to silence these sequences and therefore display epigenetic modifications such as methylation of DNA and (K9)H3 also within euchromatic regions.

引用

页码：967 / 973

页数：7

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