Intranasal immunization with a proteoliposome-derived cochleate containing recombinant gD protein confers protective immunity against genital herpes in mice

被引：42

作者：

Del Campo, Judith ^{[2
]}

Lindqvist, Madelene ^{[1
]}

Cuello, Maribel ^{[2
]}

Backstrom, Malin ^{[3
]}

Cabrerra, Osmir ^{[2
]}

Persson, Josefine ^{[1
]}

Perez, Oliver ^{[2
]}

Harandi, Ali M. ^{[1
]}

机构：

[1] Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Microbiol & Immunol, Gothenburg, Sweden

[2] Finlay Inst, Dept Immunol, Havana, Cuba

[3] Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Med Biochem & Cell Biol,Mammalian Prot Expre, Gothenburg, Sweden

来源：

VACCINE | 2010年 / 28卷 / 05期

基金：

瑞典研究理事会;

关键词：

Genital herpes; Sexually transmitted infections; Adjuvant; Toll like receptor; Mucosal immunity; Mucosal vaccine; SIMPLEX-VIRUS TYPE-2; CPG OLIGODEOXYNUCLEOTIDES; GAMMA-INTERFERON; T-CELLS; INFECTION; ADJUVANT; DELIVERY; VACCINES; RESPONSES; INNATE;

D O I：

10.1016/j.vaccine.2009.11.035

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The purpose of this study was to investigate the potential of intranasal (IN) immunization with Neisseria meningitides B proteoliposome (AFPL1) and AFPL1-derived cochleate (AFCo1), containing glycoprotein D (gD) of herpes simplex virus type 2 (HSV-2) for induction of protective immunity against genital herpes infection in mice. We could show that IN immunization with both AFPL1 and AFCo1 containing gD induced gD-specific IgG antibody and lymphoproliferative responses. However, IFN-gamma response could only be detected in CD4(+) splenic cells and genital lymph node cells of the AFCo1gD immunized mice upon recall antigen stimulation in vitro. Importantly, IN immunization with AFCo1gD could elicit a complete protection against an otherwise lethal vaginal challenge with HSV-2, while the AFPL1gD immunized mice were only partially protected. Further, we could show that the IFN-gamma response and protective immunity observed after IN immunization with AFCo1gD are mediated via the adaptor molecule myeloid differentiation factor 88. These data may have implications for the development of a mucosal vaccine against genital herpes. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1193 / 1200

页数：8

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