Polypyrrole DNA chip on a silicon device:: Example of hepatitis C virus genotyping

被引:164
作者
Livache, T
Fouque, B
Roget, A
Marchand, J
Bidan, G
Téoule, R
Mathis, G
机构
[1] CIS Bio Int, DIVT, F-30203 Bagnols Ceze, France
[2] Univ Grenoble 1, CEA Grenoble, Dept Rech Fondamentale Mat Condensee, CNRS,UMR 585, F-38054 Grenoble 9, France
关键词
D O I
10.1006/abio.1997.2462
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We describe in this article an oligonucleotide array constructed on a silicon device bearing a matrix of addressable 50-mu m microelectrodes. Each electrode was covered by a conducting polymer (polypyrrole) grafted by an oligonucleotide (ODN). The DNA chip was prepared by successive electrochemically addressed copolymerizations of 5' pyrrole-labeled ODN and pyrrole. Following hybridization of the biotinylated amplified sample on the chip bearing a series of probes, detection was carried out by fluorescence microscopy through an R-phycoerythrin label. This technology was successfully applied to the genotyping of hepatitis C virus in blood samples. Results show good sensitivity and a high degree of dimensional resolution. (C) 1998 Academic Press.
引用
收藏
页码:188 / 194
页数:7
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