Fabrication of poly(vinyl alcohol)-Carrageenan scaffolds for cryopreservation: Effect of composition on cell viability

被引：41

作者：

Chopra, Pankaj ^{[1
]}

Nayak, Debasis ^{[2
]}

Nanda, Arpita ^{[2
]}

Ashe, Sarbani ^{[2
]}

Rauta, Pradipta Ranjan ^{[2
]}

Nayak, Bismita ^{[2
]}

机构：

[1] Thapar Univ, Dept Biotechnol, Patiala 147004, Punjab, India

[2] Natl Inst Technol, Dept Life Sci, Immunol & Mol Med Lab, Rourkela 769008, Odisha, India

来源：

CARBOHYDRATE POLYMERS | 2016年 / 147卷

关键词：

Scaffolds; Biodegradable; Biocompatible; Cell attachment; Cryopreservation; TISSUE ENGINEERING APPLICATIONS; IN-VITRO; CONSTRUCTS; HYDROGELS; ALGINATE;

D O I：

10.1016/j.carbpol.2016.04.027

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The present investigation reports the fabrication of three dimensional (3D), interconnected, highly porous, biodegradable scaffolds using freeze-gelation technique. The hydrogels prepared with different ratios (5:5, 6:4, 7:3, 8:2 and 9:1) of poly(vinyl alcohol) (PVA) and Carrageenan (Car) was lyophilized to obtain their respective scaffolds. The PVA-Car scaffolds were further characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The prepared scaffolds were found to be biodegradable and highly compatible with hemoglobin. Further, normal keratinocyte (HaCaT) and osteosarcoma (Saos-2) cells seeded on PVA-Car scaffolds were cryopreserved for 15 days and their viability was checked at regular interval of 3 days (0, 3, 6, 9, 12, 15 days) through MTT assay and fluorescence microscopy. Overall, the collective results indicate the scaffold constructs with 7:3 and 8:2 PVA-Car ratios possess ideal characteristics for tissue engineering applications and for long term cryopreservation of cells. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：509 / 516

页数：8

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