Fabrication of poly(vinyl alcohol)-Carrageenan scaffolds for cryopreservation: Effect of composition on cell viability

被引：37
作者：
Chopra, Pankaj [1 ]
Nayak, Debasis [2 ]
Nanda, Arpita [2 ]
Ashe, Sarbani [2 ]
Rauta, Pradipta Ranjan [2 ]
Nayak, Bismita [2 ]
机构：
[1] Thapar Univ, Dept Biotechnol, Patiala 147004, Punjab, India
[2] Natl Inst Technol, Dept Life Sci, Immunol & Mol Med Lab, Rourkela 769008, Odisha, India
来源：
CARBOHYDRATE POLYMERS | 2016年 / 147卷
关键词：
Scaffolds; Biodegradable; Biocompatible; Cell attachment; Cryopreservation; TISSUE ENGINEERING APPLICATIONS; IN-VITRO; CONSTRUCTS; HYDROGELS; ALGINATE;
D O I：
10.1016/j.carbpol.2016.04.027
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The present investigation reports the fabrication of three dimensional (3D), interconnected, highly porous, biodegradable scaffolds using freeze-gelation technique. The hydrogels prepared with different ratios (5:5, 6:4, 7:3, 8:2 and 9:1) of poly(vinyl alcohol) (PVA) and Carrageenan (Car) was lyophilized to obtain their respective scaffolds. The PVA-Car scaffolds were further characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The prepared scaffolds were found to be biodegradable and highly compatible with hemoglobin. Further, normal keratinocyte (HaCaT) and osteosarcoma (Saos-2) cells seeded on PVA-Car scaffolds were cryopreserved for 15 days and their viability was checked at regular interval of 3 days (0, 3, 6, 9, 12, 15 days) through MTT assay and fluorescence microscopy. Overall, the collective results indicate the scaffold constructs with 7:3 and 8:2 PVA-Car ratios possess ideal characteristics for tissue engineering applications and for long term cryopreservation of cells. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：509 / 516
页数：8
相关论文
共 27 条
  • [1] Annabi N, 2010, TISSUE ENG PART B-RE, V16, P371, DOI 10.1089/ten.TEB.2009.0639
  • [2] Archana D, 2013, INDIAN J BIOTECHNOL, V12, P475
  • [3] Berridge MV, 2005, BIOTECHNOL ANN REV, V11, P127, DOI 10.1016/S1387-2656(05)11004-7
  • [4] Cryopreservation of hMSCs seeded silk nanofibers based tissue engineered constructs
    Bissoyi, Akalabya
    Pramanik, K.
    Panda, Niladri Nath
    Sarangi, S. K.
    [J]. CRYOBIOLOGY, 2014, 68 (03) : 332 - 342
  • [5] A critical review on polymer-based bio-engineered materials for scaffold development
    Cheung, Hoi-Yan
    Lau, Kin-Tak
    Lu, Tung-Po
    Hui, David
    [J]. COMPOSITES PART B-ENGINEERING, 2007, 38 (03) : 291 - 300
  • [6] Morphological Comparison of PVA Scaffolds Obtained by Gas Foaming and Microfluidic Foaming Techniques
    Colosi, Cristina
    Costantini, Marco
    Barbetta, Andrea
    Pecci, Raffaella
    Bedini, Rossella
    Dentini, Mariella
    [J]. LANGMUIR, 2013, 29 (01) : 82 - 91
  • [7] DANASOURI IE, 2005, MIDDLE E FERTILITY S, V10, P205
  • [8] Porous scaffold design for tissue engineering
    Hollister, SJ
    [J]. NATURE MATERIALS, 2005, 4 (07) : 518 - 524
  • [9] Structural changes of water in poly(vinyl alcohol) hydrogel during dehydration
    Kudo, Kushi
    Ishida, Junichi
    Syuu, Gika
    Sekine, Yurina
    Ikeda-Fukazawa, Tomoko
    [J]. JOURNAL OF CHEMICAL PHYSICS, 2014, 140 (04)
  • [10] Novel mechanically competent polysaccharide scaffolds for bone tissue engineering
    Kumbar, S. G.
    Toti, U. S.
    Deng, M.
    James, R.
    Laurencin, C. T.
    Aravamudhan, A.
    Harmon, M.
    Ramos, D. M.
    [J]. BIOMEDICAL MATERIALS, 2011, 6 (06)
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