Comprehensive human urine standards for comparability and standardization in clinical proteome analysis

被引：129

作者：

Mischak, Harald ^{[2
]}

Kolch, Walter ^{[3
,4
]}

Aivaliotis, Michalis

Bouyssie, David ^{[5
,6
,7
]}

Court, Magali ^{[8
]}

Dihazi, Hassan ^{[9
]}

Dihazi, Gry H. ^{[9
]}

Franke, Julia ^{[2
]}

Garin, Jerome ^{[8
]}

de Peredo, Anne Gonzalez ^{[5
,6
,7
]}

Iphoefer, Alexander ^{[10
]}

Jaensch, Lothar ^{[10
]}

Lacroix, Chrystelle ^{[5
,6
,7
]}

Makridakis, Manousos

Masselon, Christophe ^{[8
]}

Metzger, Jochen ^{[2
]}

Monsarrat, Bernard ^{[5
,6
,7
]}

Mrug, Michal ^{[11
]}

Norling, Martin ^{[12
]}

Novak, Jan ^{[11
]}

Pich, Andreas ^{[13
]}

Pitt, Andrew ^{[3
,4
]}

Bongcam-Rudloff, Erik ^{[12
]}

Siwy, Justyna ^{[2
]}

Suzuki, Hitoshi ^{[11
]}

Thongboonkerd, Visith ^{[14
]}

Wang, Li-Shun ^{[3
,4
]}

Zoidakis, Jerome

Zuerbig, Petra ^{[2
]}

Schanstra, Joost P. ^{[15
,16
]}

Vlahou, Antonia ^{[1
]}

机构：

[1] Acad Athens, Biotechnol Lab, Biomed Res Fdn, Athens 11527, Greece

[2] Mosa Diagnost & Therapeut AG, Hannover, Germany

[3] Univ Glasgow, Glasgow, Lanark, Scotland

[4] Beatson Inst Canc Res, Glasgow G61 1BD, Lanark, Scotland

[5] CNRS, IPBS, Toulouse, France

[6] Univ Toulouse, Toulouse, France

[7] UPS, Toulouse, France

[8] CEA, IRTSV, Lab Etud Dynam Proteomes, INSERM,U880, Grenoble, France

[9] Univ Gottingen, Nephrol & Rheumatol UMG, Gottingen, Germany

[10] Helmholtz Ctr Infect Res, Braunschweig, Germany

[11] Univ Alabama Birmingham, Birmingham, AL USA

[12] SLU, Linnaeus Ctr Bioinformat, Uppsala, Sweden

[13] Hannover Med Sch, Dept Pharmacol, D-3000 Hannover, Germany

[14] Mahidol Univ, Med Prote Unit, Off Res & Dev, Fac Med,Siriraj Hosp, Bangkok 10700, Thailand

[15] Fac Med Toulouse, INSERM, U858, F-31073 Toulouse, France

[16] Univ Toulouse 3, Inst Med Mol Rangueil, F-31062 Toulouse, France

来源：

PROTEOMICS CLINICAL APPLICATIONS | 2010年 / 4卷 / 04期

关键词：

Clinical proteomics; Proteome; Standard; Urine; ENHANCED LASER-DESORPTION/IONIZATION; MASS-SPECTROMETRY; BIOMARKER DISCOVERY; KIDNEY; PROTEINS; DISEASE; ELECTROPHORESIS; ESTABLISHMENT;

D O I：

10.1002/prca.200900189

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Purpose: Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study is the development of a well-characterized "real life" sample that can be used as reference standard in urine clinical proteomics studies. Experimental design: We report on the generation of male and female urine samples that are extensively characterized by different platforms and methods (CE-MS, LC-MS, LC-MS/MS, 1-D gel analysis in combination with nano-LC MS/MS (using LTQ-FT ultra), and 2-DE-MS) for their proteome and peptidome. In several cases analysis involved a definition of the actual biochemical entities, i.e. proteins/peptides associated with molecular mass and detected PTMs and the relative abundance of these compounds. Results: The combination of different technologies allowed coverage of a wide mass range revealing the advantages and complementarities of the different technologies. Application of these samples in "inter-laboratory" and "inter-platform" data comparison is also demonstrated. Conclusions and clinical relevance: These well-characterized urine samples are freely available upon request to enable data comparison especially in the context of biomarker discovery and validation studies. It is also expected that they will provide the basis for the comprehensive characterization of the urinary proteome.

引用

页码：464 / 478

页数：15

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