PHENOLIC CONTENTS AND ANTIOXIDANT ACTIVITY IN CENTRAL-SOUTHERN URUGUAYAN PROPOLIS EXTRACTS

被引:0
作者
Paulino Zunini, Margot [1 ]
Rojas, Cristhian [1 ,4 ]
De Paula, Sebastian [2 ]
Elingold, Igal [3 ]
Alvareda Migliaro, Elena [1 ]
Casanova, Marta B. [3 ]
Iribarne Restuccia, Federico [1 ]
Aguilera Morales, Sara [4 ]
Dubin, Marta [3 ]
机构
[1] Univ Republica, Fac Quim, Lab Bioinformat & Farmacoquim Mol, DETEMA, Montevideo 11600, Uruguay
[2] Univ Republica, Fac Quim, Lab Farmacogonosia & Prod Nat, DQO, Montevideo 11600, Uruguay
[3] Univ Buenos Aires, Fac Med, CONICET, CEFYBO, RA-1121 Buenos Aires, DF, Argentina
[4] Univ Catolica Norte, Fac Ciencias, Dept Fis, Antofagasta, Chile
关键词
RP-HPLC; phenols; antioxidant activity; propolis; RADICAL SCAVENGING ACTIVITY; LIPID-PEROXIDATION; QUANTITATIVE STRUCTURE; CHEMICAL-COMPOSITION; FLAVONOIDS; COMPONENTS;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A study on the phenolic content and antioxidant properties of Uruguayan propolis is presented. Identification and quantification was carried out by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Antioxidant activity was determined by Antiradical Activity Determination (AAD) measured with DPPH and Microsomal Lipid Peroxidation (MLP) assays. Two phenolic acids (gentistic and p-coumaric) and eight flavonoics (fisetin, myricetin, luteolin, quercetin, kaempferol, pinocembrin, chrysin and tectochrysin) were identified. The existence of five other phenols is postulated. The large difference between range of values of scavenger activity AAD (between 60% and 100%) as compared with lipoperoxidation inhibition (between 15% and 92%), suggests that the complexity of the biologic matrix could modulate the antioxidant effect. From a commercial standpoint, research such as the one presented here endows the product with added value, making it more appealing to the interest of industry and entrepreneurs.
引用
收藏
页码:141 / 146
页数:6
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