The target cells of injected type I interferons in mouse liver

被引:4
作者
Asada-Kubota, M [1 ]
Tatsumi, R [1 ]
Ueda, T [1 ]
Kobayashi, M [1 ]
Hamada, K [1 ]
Maekawa, S [1 ]
Sokawa, Y [1 ]
机构
[1] Kyoto Inst Technol, Dept Biotechnol, Sakyo Ku, Kyoto 606, Japan
关键词
D O I
10.1089/jir.1998.18.71
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Type I interferons (IFNs) have been used for the treatment of viral hepatitis, but it is unclear which cells in the liver are affected by injected IFN, The effects of IFN have been studied by the production of 2',5'-oligoadenylate synthetase (2'5'OAS), an IFN-inducible enzyme, Here, we studied the distribution of 2'5'OAS in mouse liver after injection of natural mouse IFN-alpha/beta by Western blotting and immunohistochemistry using a monoclonal antibody specific to mouse 42-kDa 2'5'OAS, Injection of IFN-alpha/beta increased the levels of liver 2'5'OAS and enhanced the intensities of immunohistochemical staining for this enzyme in both hepatocytes and Kupffer cells, In IFN-untreated normal mice, hepatocytes were lightly stained, but some of the Kupffer cells showed rather strong staining, The 2'5'OAS-positive Kupffer cells comprised similar to 60% of those in normal liver, whereas this increased to similar to 90% following IFN-alpha/beta injection, Thus, hepatocytes and Kupffer cells were the targets of injected IFN.
引用
收藏
页码:71 / 74
页数:4
相关论文
共 16 条
[1]   Localization of 2',5' oligoadenylate synthetase and the enhancement of its activity with recombinant interferon-alpha A/D in the mouse brain [J].
AsadaKubota, M ;
Ueda, T ;
Nakashima, T ;
Kobayashi, M ;
Shimada, M ;
Takeda, K ;
Hamada, K ;
Maekawa, S ;
Sokawa, Y .
ANATOMY AND EMBRYOLOGY, 1997, 195 (03) :251-257
[2]  
AsadaKubota M, 1996, ANAT EMBRYOL, V194, P349
[3]   DISTRIBUTION OF IMMUNOREACTIVE 2',5'-OLIGOADENYLATE SYNTHETASE IN MOUSE DIGESTIVE-TRACT [J].
ASADAKUBOTA, M ;
UEDA, T ;
SHIMADA, M ;
TAKEDA, K ;
SOKAWA, Y .
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1995, 15 (10) :863-867
[4]  
HOLLAND SP, 1991, AM J VET RES, V52, P1779
[5]  
HOOFNAGLE JH, 1992, INTERFERON PRINCIPLE, P431
[6]   PHARMACOKINETICS AND BIOLOGIC ACTIVITIES OF HUMAN NATIVE AND ASIALOINTERFERON-BETA-S [J].
KASAMA, K ;
UTSUMI, J ;
MATSUOOGAWA, E ;
NAGAHATA, T ;
KAGAWA, Y ;
YAMAZAKI, S ;
SATOH, YI .
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1995, 15 (05) :407-415
[7]  
KHAN NUD, 1989, IMMUNOLOGY, V66, P201
[8]   BIOCHEMISTRY OF INTERFERONS AND THEIR ACTIONS [J].
LENGYEL, P .
ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :251-282
[9]  
POLITIS AD, 1990, J IMMUNOL, V145, P3788
[10]   SERUM 2',5'-OLIGOADENYLATE SYNTHETASE-ACTIVITY DURING INTERFERON TREATMENT OF CHRONIC HEPATITIS-B [J].
SHINDO, M ;
OKUNO, T ;
MATSUMOTO, M ;
TAKEDA, M ;
TAKINO, T ;
SOKAWA, J ;
IWATA, A ;
SOKAWA, Y .
HEPATOLOGY, 1988, 8 (02) :366-370