Identification of active biomolecules in the high-yield synthesis of single-crystalline gold nanoplates in algal solutions

被引:257
作者
Xie, Jianping
Lee, Jim Yang
Wang, Daniel I. C.
Ting, Yen Peng
机构
[1] Natl Univ Singapore, Singapore MIT Alliance, Singapore 117576, Singapore
[2] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 119260, Singapore
[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
关键词
biosynthesis; gold; green chemistry; nanomaterials; proteins;
D O I
10.1002/smll.200600612
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this work, single-crystalline gold nanoplates were produced by treating an aqueous solution of chloroauric acid with the extract of the unicellular green alga Chlorella vulgaris at room temperature. The results suggest proteins as the primary biomolecules involved in providing the dual function of Au-III reduction and the size- and shape-controlled synthesis of the nanogold crystals. A protein with a molecular weight of approximately 28 kDa was isolated and purified by reversed-phase HPLC; this protein tested positive for the reduction of chloroauric acid in aqueous solution. The isolated protein (named gold shape-directing protein, or GSP for convenience) was then used to produce gold nanoplates with distinctive triangular and hexagonal shapes in high yields (approximate to 90 %). The kinetics of the reduction reaction could be manipulated through changes in the GSP concentration to produce plates with lateral sizes ranging from nanometers to micrometers. The growth of gold nanoplates in the GSP solution with time was monitored by microscopic and spectroscopic techniques, thereby allowing the detection of several key intermediates in the growth process.
引用
收藏
页码:672 / 682
页数:11
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