Phylogenetic analysis of plastid trnL-trnF sequences from Arisaema species (Araceae) in Korea

被引：5

作者：

Jung, YH ^{[1
]}

Song, EY ^{[1
]}

Chun, SJ ^{[1
]}

Jang, KC ^{[1
]}

Kim, M ^{[1
]}

Kang, SH ^{[1
]}

Kim, SC ^{[1
]}

机构：

[1] Rural Dev Adm, Natl Jeju Agr Expt Stn, Cheju 690150, South Korea

来源：

EUPHYTICA | 2004年 / 138卷 / 01期

关键词：

cpDNA; chloroplast DNA; MP; most-parsimonious;

D O I：

10.1023/B:EUPH.0000047078.20546.b6

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The phylogeny of 10 taxa belonging to three sections of Arisaema (Pistillata, Tortuosa, and Arisaema) distributed in Korea and an outgroup taxon (Pinellia ternate) was analyzed by comparing the trnL(UAA)-trnF(GAA) intergenic spacer sequences of the chloroplast DNA (cpDNA). The trnL-trnF regions ranged from 336 to 396 base pairs ( bp) in length. Sequence alignment required 18 base substitutions and 4 independent indels in the region. The longest length mutation was a 35-bp deletion in A. thunbergii, A. heterophyllum, A. urashima, and A. candidissimum. Two different restriction fragment patterns were seen with MspI digestions. Section Tortuosa (A. thunbergii and A. heterophyllum) plus A. urashima and A. candidissium were distinguished from the others. In addition, 16-bp deletions were found in A. thunbergii, A. heterophyllum, and A. candidissimum. Four species possessed a 24-bp insertion mutation with a duplication motif, TTTTGTTAGGTTATCCTTACACTT: A. amurense f. serratum, A. robustum f. purpureum, A. peninsulae, and A. sikokianum. A maximum parsimony analysis of 11 accessions produced 12 equally most-parsimonious ( MP) trees. The MP trees also contained three independent groups. Group I contained one taxon: A. ringens f. praecox. Group II contained the section Tortuosa accessions including A. urashima and A. candidissimum. Group III contained the section Arisaema and A. sikokianum. These results show that analyses of the cpDNA trnL-trnF intergenic spacer are a useful approach for inferring phylogenetic relationships and identification within the genus Arisaema, distributed in Korea.

引用

页码：81 / 88

页数：8

共 40 条

[1] THE ROLE OF INSERTIONS DELETIONS IN THE EVOLUTION OF THE INTERGENIC REGION BETWEEN PSBA AND TRNH IN THE CHLOROPLAST GENOME [J].

ALDRICH, J ;

CHERNEY, BW ;

MERLIN, E ;

CHRISTOPHERSON, L .

CURRENT GENETICS, 1988, 14 (02) :137-146

[2] Chloroplast-DNA variation in the wild and cultivated olives (Olea europaea L.) of Morocco [J].

Amane, M ;

Ouazzani, N ;

Lumaret, R ;

Debain, C .

EUPHYTICA, 2000, 116 (01) :59-64

[3]

[Anonymous], 1992, Molecular systematics of plants

[4] A phylogenetic study of the palm family (Palmae) based on chloroplast DNA sequences from the trnL-trnF region [J].

Baker, WJ ;

Asmussen, CB ;

Barrow, SC ;

Dransfield, J ;

Hedderson, TA .

PLANT SYSTEMATICS AND EVOLUTION, 1999, 219 (1-2) :111-126

[5] Tribal phylogeny of the Asteraceae based on two non-coding chloroplast sequences, the trnL intron and trnL/trnF intergenic spacer [J].

Bayer, RJ ;

Starr, JR .

ANNALS OF THE MISSOURI BOTANICAL GARDEN, 1998, 85 (02) :242-256

[6]

CURTIS SE, 1984, MOL BIOL EVOL, V1, P291

[7] INSERTION DELETION MUTATIONS IN THE ZEA CHLOROPLAST GENOME [J].

DOEBLEY, JF ;

MA, DP ;

RENFROE, WT .

CURRENT GENETICS, 1987, 11 (08) :617-624

[8]

DOYLE JJ, 1987, PHYTOCHEMISTRY B, V17, P144

[9]

ENGLER A, 1979, PHANEROGAMARUM, V2, P533

[10]

FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x

← 1 2 3 4 →