Generation and characterization of mice for conditional inactivation of Zeb1

被引：27
作者：
Brabletz, Simone [1 ]
Losada, Maria Lasierra [1 ]
Schmalhofer, Otto [2 ]
Mitschke, Julia [2 ]
Krebs, Angela [1 ]
Brabletz, Thomas [1 ]
Stemmler, Marc P. [1 ]
机构：
[1] Friedrich Alexander Univ Erlangen Nurnberg, Nikolaus Fiebiger Ctr Mol Med, Dept Expt Med 1, Erlangen, Germany
[2] Univ Freiburg, Fac Med, Med Ctr, Dept Gen & Visceral Surg, Freiburg, Germany
来源：
GENESIS | 2017年 / 55卷 / 04期
关键词：
cancer; Cre-loxP; epithelial-mesenchymal transition; metastasis; mouse embryogenesis; TRANSCRIPTION FACTORS; DELTA-EF1;
D O I：
10.1002/dvg.23024
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The multizinc finger containing transcription factor ZEB1 plays crucial roles during various aspects of mammalian development and tumorigenesis. Best studied in human tumors, ZEB1 is activating the embryo-derived program of epithelial-mesenchymal transition (EMT). The aberrant activation of EMT confers an invasive metastasizing phenotype with acquisition of stem cell properties and resistance to radio- and chemotherapy. Although ZEB1 has very important functions in tumor progression, not much is known about its role in physiological contexts and during development and homeostasis. We describe the generation of Zeb1(flox/flox) mice carrying a targeted mutation for conditional Zeb1 gene inactivation and show that homozygous Zeb1-depletion in the germline results in a phenotype similar to the conventional Zeb1 knockout.
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页数：7
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