Tumour necrosis factor gene polymorphisms in Egyptian patients with rheumatoid arthritis and their relation to disease activity and severity

被引:6
作者
Zaghlol, Heba M. [1 ]
Abdelshafy, Sanaa [1 ]
Mohamed, Rabab A. [1 ]
Abdelaleem, Enas A. [2 ]
机构
[1] Beni Suef Univ, Dept Clin Pathol, Fac Med, Bani Suwayf, Egypt
[2] Beni Suef Univ, Fac Med, Dept Rheumatol & Rehabil, Bani Suwayf 002082, Egypt
关键词
rheumatoid arthritis; genetic susceptibility; TNF polymorphisms; polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP); FACTOR-ALPHA; TNF-ALPHA; PROMOTER POLYMORPHISM; RADIOGRAPHIC PROGRESSION; SUSCEPTIBILITY; ASSOCIATION; POPULATION; BIOLOGY; LEVEL; IL-1;
D O I
10.5114/ceji.2019.89602
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Aim of the study: The present case control study was conducted to assess the association of LTA 252 A>G, TNF-alpha 308 G>A, and TNF-alpha 1031 T>C gene polymorphisms with rheumatoid arthritis (RA), and their involvement in disease activity and severity. Material and methods: A total of 70 Egyptians, including 35 RA patients and 35 healthy control individuals, were included in the study. The RA patients comprised 34 females and one male. Cases with RA were diagnosed by a rheumatologist and fulfilled the 2010 ACR/EULAR criteria. Modified disease activity score (DAS28) was used to assess disease activity. Van Der Heijde-modified Sharp score (rRES) was used to assess radiological changes for assessment of disease severity. PCR-RFLP was used to detect the association of LTA 252 A>G, TNF-alpha 308 G>A, and TNF-alpha 1031 T>C gene polymorphisms with RA. Results: TNF-alpha 308 G allele and TNF-alpha 308 GG genotype were significantly higher in RA patients compared to healthy control subjects (p = 0.04 and p = 0.001, respectively). TNF-alpha 308 G allele and GG genotype were significantly higher in the RA non-remission group compared to the remission group (p = 0.008, p < 0.001). Patients with the TNF-alpha 308 AG genotype had higher mean of Sharp score compared to the patients with the GG and AA genotypes (p = 0.007). There was no significant association between LTA 252 A>G and TNF-alpha 1031 T>C gene polymorphisms and R4. Conclusions: Our results suggest that TNF-alpha 308 G/A gene polymorphism is genetically associated with RA and involved in disease activity and severity in Egyptian patients.
引用
收藏
页码:277 / 284
页数:8
相关论文
共 49 条
[1]  
ABRAHAM LJ, 1993, CLIN EXP IMMUNOL, V92, P14
[2]   TNF-alpha and TNF-beta Gene Polymorphism in Saudi Rheumatoid Arthritis Patients [J].
Al-Rayes, Hannan ;
Al-Swailem, Ramiz ;
Albelawi, Maysson ;
Arfin, Misbahul ;
Al-Asmari, Abdulrahman ;
Tariq, Mohammad .
CLINICAL MEDICINE INSIGHTS-ARTHRITIS AND MUSCULOSKELETAL DISORDERS, 2011, 4 :55-63
[3]  
Aletaha D, 2010, ANN RHEUM DIS, V69, P1580, DOI [10.1136/ard.2010.138461, 10.1002/art.27584]
[4]   SLE - Complex cytokine effects in a complex autoimmune disease: tumor necrosis factor in systemic lupus erythematosus [J].
Aringer, M ;
Smolen, JS .
ARTHRITIS RESEARCH & THERAPY, 2003, 5 (04) :172-177
[5]   Tumor necrosis factor-alpha and Interleukin-10 gene promoter polymorphisms in Turkish rheumatoid arthritis patients [J].
Ates, Omer ;
Hatemi, Gulen ;
Hamuryudan, Vedat ;
Topal-Sarikaya, Aysegul .
CLINICAL RHEUMATOLOGY, 2008, 27 (10) :1243-1248
[6]  
Avise John C., 1994, pi
[7]  
Balog A., 2004, Acta Microbiologica et Immunologica Hungarica, V51, P263, DOI 10.1556/AMicr.51.2004.3.4
[8]   Polymorphisms in the tumour necrosis factor gene are not associated with severity of inflammatory polyarthritis [J].
Barton, A ;
Platt, H ;
Salway, F ;
Symmons, D ;
Barrett, E ;
Bukhari, M ;
Lunt, M ;
Zeggini, E ;
Eyre, S ;
Hinks, A ;
Tellam, D ;
Brintnell, B ;
Ollier, W ;
Worthington, J ;
Silman, A .
ANNALS OF THE RHEUMATIC DISEASES, 2004, 63 (03) :280-284
[9]   THE BIOLOGY OF CACHECTIN/TNF - A PRIMARY MEDIATOR OF THE HOST RESPONSE [J].
BEUTLER, B ;
CERAMI, A .
ANNUAL REVIEW OF IMMUNOLOGY, 1989, 7 :625-655
[10]   TNF-α gene polymorphisms in Iranian Azeri Turkish patients with Behcet's Disease [J].
Bonyadi, Mortaza ;
Jahanafrooz, Zohreh ;
Esmaeili, Mohsen ;
Kolahi, Susan ;
Khabazi, Alireza ;
Ebrahimi, Ali Asghar ;
Hajialilo, Mehrzad ;
Dastgiri, Saeed .
RHEUMATOLOGY INTERNATIONAL, 2009, 30 (02) :285-289