Berberine attenuates choline-induced atherosclerosis by inhibiting trimethylamine and trimethylamine-N-oxide production via manipulating the gut microbiome

被引：128

作者：

Li, Xingxing ^{[1
,2
]}

Su, Chunyan ^{[1
,2
]}

Jiang, Zhibo ^{[1
]}

Yang, Yuxin ^{[1
]}

Zhang, Yue ^{[1
]}

Yang, Mengxia ^{[1
]}

Zhang, Xiumin ^{[1
]}

Du, Yu ^{[1
]}

Zhang, Jin ^{[1
]}

Wang, Li ^{[1
]}

Jiang, Jiandong ^{[1
]}

Hong, Bin ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, NHC Key Lab Biotechnol Antibiot, Beijing, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, CAMS Key Lab Synthet Biol Drug Innovat, Beijing, Peoples R China

来源：

NPJ BIOFILMS AND MICROBIOMES | 2021年 / 7卷 / 01期

基金：

中国国家自然科学基金;

关键词：

BILE-ACID METABOLISM; INFLAMMATION; ACTIVATION; CARNITINE; TMAO; DIET;

D O I：

10.1038/s41522-021-00205-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Trimethylamine-N-oxide (TMAO), a derivative from the gut microbiota metabolite trimethylamine (TMA), has been identified to be an independent risk factor for promoting atherosclerosis. Evidences suggest that berberine (BBR) could be used to treat obesity, diabetes and atherosclerosis, however, its mechanism is not clear mainly because of its poor oral bioavailability. Here, we show that BBR attenuated TMA/TMAO production in the C57BL/6J and ApoE KO mice fed with choline-supplemented chow diet, and mitigated atherosclerotic lesion areas in ApoE KO mice. Inhibition of TMA/TMAO production by BBR-modulated gut microbiota was proved by a single-dose administration of d9-choline in vivo. Metagenomic analysis of cecal contents demonstrated that BBR altered gut microbiota composition, microbiome functionality, and cutC/cntA gene abundance. Furthermore, BBR was shown to inhibit choline-to-TMA conversion in TMA-producing bacteria in vitro and in gut microbial consortium from fecal samples of choline-fed mice and human volunteers, and the result was confirmed by transplantation of TMA-producing bacteria in mice. These results offer new insights into the mechanisms responsible for the anti-atherosclerosis effects of BBR, which inhibits commensal microbial TMA production via gut microbiota remodeling.

引用

页数：14

共 66 条

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