Analysis of neurotoxin 3-N-oxalyl-L-2,3-diaminopropionic acid and its α-isomer in Lathyrus sativus by high-performance liquid chromatography with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization

被引：28
作者：
Chen, X
Wang, F
Chen, Q
Qin, XC
Li, ZX [1 ]
机构：
[1] Lanzhou Univ, Natl Lab Appl Organ Chem, Gansu 730000, Peoples R China
[2] Lanzhou Univ, State Key Lab Arid Agroecol, Gansu 730000, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 08期
关键词：
RP HPLC; neurotoxin; Lathyrus sativus; 6-aminoquinolyl-N-hydroxysuccininimidyl carbamate;
D O I：
10.1021/jf000033y
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A method was developed for the quantitative determination of the neurotoxic nonprotein amino acid, 3-N-oxalyl-L-2,3-diaminopropionic acid (beta-ODAP), and its nontoxic alpha-isomer, 2-N-oxalyl-L-2,3-diaminopropionic acid (alpha-ODAP), in the plant samples of Lathyrus sativus after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) by reversed-phase high-performance liquid chromatography (HPLC). Hippuric acid was used as an internal standard. A linear response was recorded in the concentration rang 0.32-32 nmol with r > 0.999. The RP HPLC detection limit for both isomers is 1.8 ng. According to different experimental needs, a ternary gradient system can be used to determine toxin and other nonprotein amino acids. The RP HPLC method and a colorimetric method were compared for measuring ODAP.
引用
收藏
页码：3383 / 3386
页数：4
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