Development and Validation of an LC-MS/MS Method for the Quantification of Agaritine in Mushrooms

被引：3

作者：

Merdivan, Simon ^{[1
]}

Willke, Christoph ^{[1
]}

Lindequist, Ulrike ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Inst Pharm, Dept Pharmaceut Biol, Felix Hausdorff Str 1, D-17487 Greifswald, Germany

来源：

INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS | 2016年 / 18卷 / 01期

关键词：

medicinal mushrooms; agaritine; LC-MS/MS; Agaricus brasiliensis; Agaricus bisporus; AGARICUS-BLAZEI MURRILL; ANTITUMOR-ACTIVITY; BISPORUS; MICE; DERIVATIVES; HYDRAZINE; INDUCTION; MURILL;

D O I：

10.1615/IntJMedMushrooms.v18.i1.30

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agaritine, an aromatic hydrazine, is found as a secondary metabolite in mushroom species. It is among others suspected to exhibit genotoxic activity. This publication describes the validation of a method for the quantification of agaritine in mushrooms (i.e., extraction and purification by solid phase extraction) and measurement by liquid chromatography with tandem mass spectrometry detection in positive ionization mode. The results show this method to be selective, accurate, and precise. This method could be used for the quality control of pharmaceutical preparations containing mushrooms.

引用

页码：13 / 21

页数：9

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