Genetic approaches to retinal research in zebrafish

被引:11
作者
Niklaus, Stephanie [1 ,2 ]
Neuhauss, Stephan C. F. [1 ]
机构
[1] Univ Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
[2] Life Sci Zurich Grad Program Neurosci, Zurich, Switzerland
基金
瑞士国家科学基金会;
关键词
Retina; reverse genetics; forward genetics; zebrafish; transgenesis; PHOTOPIC SPECTRAL SENSITIVITY; SITE-SPECIFIC RECOMBINATION; ZINC-FINGER NUCLEASES; DANIO-RERIO RETINA; TRANSGENIC ZEBRAFISH; BRACHYDANIO-RERIO; INSERTIONAL MUTAGENESIS; FLUORESCENT PROTEIN; NEURAL CIRCUITS; VISUAL-SYSTEM;
D O I
10.1080/01677063.2017.1343316
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The zebrafish (Danio rerio) possesses a vertebrate-type retina that is extraordinarily conserved in evolution. This well-organized and anatomically easily accessible part of the central nervous system has been widely investigated in zebrafish, promoting general understanding of retinal development, morphology, function and associated diseases. Over the recent years, genome and protein engineering as well as imaging techniques have experienced revolutionary advances and innovations, creating new possibilities and methods to study zebrafish development and function. In this review, we focus on some of these emerging technologies and how they may impact retinal research in the future. We place an emphasis on genetic techniques, such as transgenic approaches and the revolutionizing new possibilities in genome editing.
引用
收藏
页码:70 / 87
页数:18
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